Purification of Mitochondria by Sucrose Step Density Gradient Centrifugation
- 1Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147-2408;
- 2Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06520-8023;
- 3Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06520-8023
Abstract
Mitochondrial fractions isolated from tissue culture cells or tissue such as liver after differential centrifugation can be purified further by density gradient centrifugation. Here we describe the use of sucrose for this purpose because it is commonly used and inexpensive and the resulting mitochondria preparations are useful for many purposes.
Footnotes
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↵4 Correspondence: gerald.shadel{at}yale.edu
- © 2014 Cold Spring Harbor Laboratory Press
