Generation of High-Titer Lentivirus for the Production of Transgenic Quail
- Division of Biology and the Biological Imaging Center, Beckman Institute, California Institute of Technology, Pasadena, CA 91125, USA
- ↵1Corresponding author (rusty{at}caltech.edu)
INTRODUCTION
This protocol describes how to generate high-titer lentivirus for the production of transgenic Japanese quail. The virus is pseudotyped with vesicular stomatitis virus with the envelope G glycoprotein (VSV-g), which gives a broad infectious range and allows concentration of viral supernatants by ultracentrifugation. Using this method, we typically produce titers >1 × 108 transforming units (TU)/mL and recommend using a virus with a titer at least this high for in vivo work.
