Injection of Lentivirus into Stage-X Blastoderm for the Production of Transgenic Quail
- Division of Biology and the Biological Imaging Center, Beckman Institute, California Institute of Technology, Pasadena, CA 91125, USA
- ↵1Corresponding author (rusty{at}caltech.edu)
INTRODUCTION
This protocol describes how to generate transgenic quail by injecting a lentiviral vector into freshly laid Japanese quail eggs at stage X. The lentivirus infects primordial germ cells originating in the area pellucida.
