The In Vivo Distribution of Bacterial Polysomes, Ribosomes, and Ribosomal Subunits
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Excerpt
Two types of ribosomal distributions have been reported for bacteria by various investigators who employed different monovalent cations in the lysis media and/or gradients: a type-I distribution, which consists of polysomes, little or no 70 S monomers, and 50 S and 30 S ribosomal subunits, has been obtained from Escherichia coli lysates in TM-buffer (0.005 M Tris, pH 7.4 at 5°C with 10 mM Mg#x002B;2) with 40 to 60 mM NaCl (Mangiarotti et al., 1966; Mangiarotti and Schlessinger, 1966, 1967; Phillips et al., 1969a). A type-II distribution, which consists of polysomes, a prominent peak of 70 S monomers, and 50 S and 30 S ribosomal subunits has been obtained from Escherichia coli lysates in TM-buffer with 60 to 100 mM KCl (Ennis, 1968; Phillips et al., 1969a; MacDonald and Yeater, 1968; Kohler et al., 1968; Ron et al., 1968; Subramanian et al., 1968; Godson and Sinsheimer, 1967; Algranati et al.,...
