Extent of Terminal Repetition in Adenovirus 2 DNA
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Excerpt
The adenovirus 2 (Ad-2) genome is a linear duplex DNA of molecular weight about 23 × 106 (Green et al. 1967). Denaturation and renaturation of this DNA at low concentration leads to the formation of single-stranded circles observable by electron microscopy (Garon et al. 1972; Wolfson and Dressler 1972). This phenomenon has also been seen in the DNA of adenovirus serotypes 1, 3, 7, 18 and 31 (Garon et al. 1972) and adeno-associated virus (Koczot et al. 1973; Berns and Kelly 1974). These observations led to the suggestion that the genome possesses an inverted terminal repetition and that the circular structures contain “panhandles” (Fig. 1). Studies on the extent of exonuclease III digestion necessary to abolish circle formation suggested that the terminal repetition was greater than 350 nucleotide pairs long (Garon et al. 1972). A second estimate of 200–500 nucleotide pairs was based on consideration of the heat stability of...
