Metabolism of mRNA from the Transforming Region of Adenovirus 2
This extract was created in the absence of an abstract.
Excerpt
To determine in mammalian cells the level at which control operates for any gene or group of genes, the structural organization of these genes must be defined and a number of functional variables of mRNA metabolism must be examined (Darnell 1979): (1) The boundaries of the transcription unit must be defined so that the rate of synthesis of primary transcripts including the mRNA sequences can be estimated. (2) The efficiency of nuclear processing and cytoplasmic appearance of an mRNA must be determined, that is, does every primary transcriptional product containing the sequences for a particular mRNA actually give rise to that mRNA in the cytoplasm? (3) A new demand on studies that aim to describe gene control in animal cells has arisen because of the discovery of the splicing of primary gene transcripts. If a primary transcript has two or more different mRNAs but only one cap or leader sequence,...
