Isolation of a polA Mutation That Affects Transposition of Insertion Sequences and Transposons
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Excerpt
The bacterial transposable elements include the insertion sequences and the transposons. In addition to undergoing transposition, these elements mediate a number of other genetic rearrangements, including deletions, inversions, and inverted duplications (Botstein and Kleckner 1977; Kleckner et al. 1979; Ross et al. 1979; Syvanen 1980). They excise precisely or imprecisely in a reaction that appears to be independent of the transposition reaction (Rubens et al. 1976; Berg 1977).
Genetic studies of transposable elements in E. coli have focused primarily on the elements themselves. The transposons Tn3 (Chou et al. 1979; Gill et. al. 1979) and Tn5 (Rothstein et al. 1980) have been shown to carry structural genes for proteins required for their own transposition. Little has been discovered, however, about host functions involved in transposition. A number of host functions that interact with other transposon-mediated genetic rearrangements have been identified. A mutation designated del (Nevers and Saedler 1978) reduces the...
