Studies on DNA Replication in the Bacteriophage T4 a--.gif System
This extract was created in the absence of an abstract.
Excerpt
In vitro systems reconstituted from the purified components of a DNA replication apparatus have provided us with much of our knowledge about the mechanistic aspects of DNA replication. The large DNA bacteriophages are especially suited for this approach, since they code for most of the proteins required for their own replication. This makes the task of defining essential replication genes and isolating their products much more straightforward than in bacteria or eukaryotic cells.
Extensive genetic analysis of bacteriophage T4 has identified 11 genes whose products appear to be directly involved in the formation and subsequent movement of DNA replication forks: genes 32, 39, 41, 43, 44, 45, 52, 60, 61, 62, and dda (Epstein et al. 1964; Warner and Hobbs 1967; Curtis and Alberts 1976; McCarthy et al. 1976; P. Gauss et al., pers. comm.). The proteins specified by these genes have been isolated and purified to near homogeneity in...
