Recent advances in single-molecule experiments show that various complex systems display nonergodic behavior. In this paper, we show how to test ergodicity and ergodicity breaking in experimental data. Exploiting the so-called dynamical functional, we introduce a simple test which allows us to verify ergodic properties of a real-life process. The test can be applied to a large family of stationary infinitely divisible processes. We check the performance of the test for various simulated processes and apply it to experimental data describing the motion of mRNA molecules inside live Escherichia coli cells. We show that the data satisfy necessary conditions for mixing and ergodicity. The detailed analysis is presented in the supplementary material.

• Received 21 July 2011

DOI:https://doi.org/10.1103/PhysRevE.84.051138