Formation of Supported Membranes from Vesicles

C. A. Keller; K. Glasmästar; V. P. Zhdanov; B. Kasemo

doi:10.1103/PhysRevLett.84.5443

Formation of Supported Membranes from Vesicles

C. A. Keller, K. Glasmästar, V. P. Zhdanov, and B. Kasemo

Phys. Rev. Lett. 84, 5443 – Published 5 June 2000

Abstract

Using a combination of the quartz crystal microbalance and surface plasmon resonance techniques, we have studied the spontaneous formation of supported lipid bilayers from small ( $\sim 25 nm$ ) unilamellar vesicles. Together these experimental methods measure the amount of lipid adsorbed on the surface and the amount of water trapped by the lipid. With this approach, we have, for the first time, been able to observe in detail the progression from the adsorption of intact vesicles to rupture and bilayer formation. Monte Carlo simulations reproduce the data.

Received 14 October 1999

DOI:https://doi.org/10.1103/PhysRevLett.84.5443

Authors & Affiliations

C. A. Keller¹, K. Glasmästar¹, V. P. Zhdanov^1,2, and B. Kasemo¹

¹Department of Applied Physics, Chalmers University of Technology and Göteborg University, S-412 96 Göteborg, Sweden
²Boreskov Institute of Catalysis, Russian Academy of Sciences, Novosibirsk 630090, Russia