DNA electrophoresis in dilute polymer solutions: A nonbinary mechanism

Axel Ekani-Nkodo and Bernard Tinland
Phys. Rev. E 67, 051920 – Published 21 May 2003
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Abstract

The dynamical behavior of the neutral polymer (dextran, Mw=2×106) is investigated during DNA electrophoresis in a dilute solution. Using a fluorescence recovery after photobleaching setup, we measured the velocity of fluorescein-labeled dextran induced by the migration of the DNA. We found that each DNA molecule drags a large number of dextrans with it. We show that DNA-dextran interactions are not only binary but long range and indirect. We conclude that the DNA-dextran complex creates a hydrodynamic field that entrains polymers far from the DNA during electrophoresis.

  • Received 7 November 2002

DOI:https://doi.org/10.1103/PhysRevE.67.051920

©2003 American Physical Society

Authors & Affiliations

Axel Ekani-Nkodo* and Bernard Tinland

  • Institut Charles Sadron-CNRS, 6 rue Boussingault, 67083 Strasbourg Cedex, France

  • *Corresponding author. Email address: tinland@ics.u-strasbg.fr
  • Present address: Physics Department, University of California at Santa Barbara, Santa Barbara, CA 93106.

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Vol. 67, Iss. 5 — May 2003

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