Figure 1

(Color) The complete unzipping and subsequent rezipping of $\lambda$-DNA in the presence of $1\mathrm{pM}$ (blue crosses) and $70\mathrm{nM}$ (red circles) E. Coli SSB proteins. Inset: complete (green crosses) and partial (purple circles) unzipping of $\lambda$-DNA in the presence of $140\mathrm{nM}$ E. Coli SSB protein.Reuse & Permissions

Figure 2

Unzipping force measured in the presence of E. Coli SSB proteins as a function of concentration (circles). The range of measured forces is due to a spread in the magnetic beads. The dashed line through the circles represents a best-fit line to the data. The triangles represent the ratio of rezipping force to unzipping force as a function of E. Coli SSB protein concentration. The dashed line through the triangles represents a best-fit to the data from Eq. (1) with $C^{-1}=2\times {10}^9{\mathrm{M}}^{-1}$ and a slope factor of 0.6. The right $y$ axis corresponds to the unzipping force, while the left $y$ axis corresponds to the ratio of rezipping force to unzipping force.Reuse & Permissions

Figure 3

(Color) Complete unzipping and rezipping in the presence of $2\mathrm{nM}$ (blue plusses), $5\mathrm{nM}$ (green crosses), and $8\mathrm{nM}$ (red circles) T4 gp32.Reuse & Permissions

Figure 4

The unzipping force measured in the presence of T4 gp32 as a function of protein concentration (circles). The dashed line through the circles represents a best-fit line to the data. No significant concentration trend is observed within the resolution of the experiment. The triangles represent the ratio of rezipping force to unzipping force in the presence of T4 gp32 as a function of concentration. The large error bars at the higher concentrations ($8\mathrm{nM}$ and greater) are indicative of the difficulty in measuring a rezipping force when little to no rezipping occurs. The dashed line through the triangles represents a best fit to the data from Eq. (1) with $C^{-1}=2\times {10}^8{\mathrm{M}}^{-1}$ and a slope factor of 10.3. The right $y$ axis corresponds to the unzipping force, while the left $y$ axis corresponds to ratio of rezipping force to unzipping force.Reuse & Permissions