Detection of harmful algal bloom causing microalgae using covalently immobilised capture oligonucleotide probes on glass and poly(dimethylsiloxane) surfaces

Karen L. Bruce; Amanda V. Ellis; Sophie C. Leterme; Dmitriy A. Khodakov; Claire E. Lenehan

doi:10.1117/12.2034011

7 December 2013 Detection of harmful algal bloom causing microalgae using covalently immobilised capture oligonucleotide probes on glass and poly(dimethylsiloxane) surfaces

Karen L. Bruce, Amanda V. Ellis, Sophie C. Leterme, Dmitriy A. Khodakov, Claire E. Lenehan

Author Affiliations +

Proceedings Volume 8923, Micro/Nano Materials, Devices, and Systems; 89235B (2013) https://doi.org/10.1117/12.2034011
Event: SPIE Micro+Nano Materials, Devices, and Applications, 2013, Melbourne, Victoria, Australia

Abstract

Harmful algal bloom (HAB) events have been on the rise in the last few decades with some of the causative microalgae exhibiting toxic properties. Therefore, detection is essential in order to prevent mortality of aquatic life and poisoning events from consumption of these biotoxins. Here, oligonucleotide modified glass and poly(dimethylsiloxane) (PDMS) surfaces have been developed for the detection of the HAB causing microalgae, Alexandrium catenella, in a model system. Our preliminary studies show that the glass surface offers superior stability and analytical response when compared to those prepared from PDMS.

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Karen L. Bruce, Amanda V. Ellis, Sophie C. Leterme, Dmitriy A. Khodakov, and Claire E. Lenehan "Detection of harmful algal bloom causing microalgae using covalently immobilised capture oligonucleotide probes on glass and poly(dimethylsiloxane) surfaces", Proc. SPIE 8923, Micro/Nano Materials, Devices, and Systems, 89235B (7 December 2013); https://doi.org/10.1117/12.2034011

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