Adaptive spatial filtering for off-axis digital holographic microscopy based on region recognition approach with iterative thresholding

Xuefei He; Chuong Vinh Nguyen; Mrinalini Pratap; Yujie Zheng; Yi Wang; David R. Nisbet; Melanie Rug; Alexander G. Maier; Woei Ming Lee

doi:10.1117/12.2242876

9 December 2016 Adaptive spatial filtering for off-axis digital holographic microscopy based on region recognition approach with iterative thresholding

Xuefei He, Chuong Vinh Nguyen, Mrinalini Pratap, Yujie Zheng, Yi Wang, David R. Nisbet, Melanie Rug, Alexander G. Maier, Woei Ming Lee

Author Affiliations +

Proceedings Volume 10013, SPIE BioPhotonics Australasia; 1001329 (2016) https://doi.org/10.1117/12.2242876
Event: SPIE BioPhotonics Australasia, 2016, Adelaide, Australia

Abstract

Here we propose a region-recognition approach with iterative thresholding, which is adaptively tailored to extract the appropriate region or shape of spatial frequency. In order to justify the method, we tested it with different samples and imaging conditions (different objectives). We demonstrate that our method provides a useful method for rapid imaging of cellular dynamics in microfluidic and cell cultures.

Citation Download Citation

Xuefei He, Chuong Vinh Nguyen, Mrinalini Pratap, Yujie Zheng, Yi Wang, David R. Nisbet, Melanie Rug, Alexander G. Maier, and Woei Ming Lee "Adaptive spatial filtering for off-axis digital holographic microscopy based on region recognition approach with iterative thresholding", Proc. SPIE 10013, SPIE BioPhotonics Australasia, 1001329 (9 December 2016); https://doi.org/10.1117/12.2242876

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available