Simulation of the stimulated Raman scattering signal generation in scattering media excited by Bessel beams

Xinyu Wang; Yonghua Zhan; Jimin Liang; Xueli Chen

doi:10.1117/12.2508494

4 March 2019 Simulation of the stimulated Raman scattering signal generation in scattering media excited by Bessel beams

Xinyu Wang, Yonghua Zhan, Jimin Liang, Xueli Chen

Author Affiliations +

Proceedings Volume 10890, Label-free Biomedical Imaging and Sensing (LBIS) 2019; 108900R (2019) https://doi.org/10.1117/12.2508494
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Stimulated Raman scattering (SRS) microscopy has been increasingly used in biology and medicine in recent years due to its ability to image chemical bonds without labelling. Traditional SRS imaging uses Gaussian beams as the excitation sources, which can achieve high spatial and axial resolutions because of the tight focus of the Gaussian beam. However, the tight focus poses serious problems for observing the scattering media. The Gaussian beam would defocus after propagating through a small distance in scattering media. The SRS microscopy cannot work well in this case. Having the self-healing property, Bessel beams may bring solution to this problem. In our previous work, we applied the Bessel beams to the SRS and implemented three-dimensional SRS imaging with projection concept. Here, we simulated the propagation of Bessel beams and the generation of SRS signals with the beam propagation method (BPM). By adding glass beads on the beam propagation path to simulate scatters, the propagation of the Bessel beams and the generation of the SRS signals would change. We designed a series of simulations to investigate the influence of the size and position of the added glass beads to the generation of SRS signals. Simulation results demonstrated that the SRS signals can generate or be recovered at the certain depth in scattering media.

Citation Download Citation

Xinyu Wang, Yonghua Zhan, Jimin Liang, and Xueli Chen "Simulation of the stimulated Raman scattering signal generation in scattering media excited by Bessel beams", Proc. SPIE 10890, Label-free Biomedical Imaging and Sensing (LBIS) 2019, 108900R (4 March 2019); https://doi.org/10.1117/12.2508494

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available