Characterization of multiphoton laser scanning device optical parameters for image restoration

Frank Fischer; Karsten Konig; Stefan Puschmann; Roger Wepf; Iris Riemann; Volker Ulrich; Peter Fischer

doi:10.1117/12.545604

8 September 2004 Characterization of multiphoton laser scanning device optical parameters for image restoration

Frank Fischer, Karsten Konig, Stefan Puschmann, Roger Wepf, Iris Riemann, Volker Ulrich, Peter Fischer

Proceedings Volume 5463, Femtosecond Laser Applications in Biology; (2004) https://doi.org/10.1117/12.545604
Event: Photonics Europe, 2004, Strasbourg, France

Abstract

Fluorescent nanobeads embedded in agarose and skin biopsies were used to optically characterize spatial and temporal resolution of multiphoton laser scanning devices (MPLSD). Optical sections based on two-photon excited bead fluorescence have been performed at various sample depths. Three-dimensional reconstruction of the image stacks allowed determination of the point spread function. Using calculated point spread functions to apply deconvolution procedures (e.g. Huygens software), the visualization and hence the interpretation of intradermal structures, such as extracellular matrix components in 150 μm tissue depth, was improved.

Citation Download Citation

Frank Fischer, Karsten Konig, Stefan Puschmann, Roger Wepf, Iris Riemann, Volker Ulrich, and Peter Fischer "Characterization of multiphoton laser scanning device optical parameters for image restoration", Proc. SPIE 5463, Femtosecond Laser Applications in Biology, (8 September 2004); https://doi.org/10.1117/12.545604

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