FTIR spectroscopy for bacterial spore identification and classification

Nancy B. Valentine; Timothy J. Johnson; Yin-Fong Su; Joel B. Forrester

doi:10.1117/12.686232

25 October 2006 FTIR spectroscopy for bacterial spore identification and classification

Nancy B. Valentine, Timothy J. Johnson, Yin-Fong Su, Joel B. Forrester

Proceedings Volume 6378, Chemical and Biological Sensors for Industrial and Environmental Monitoring II; 63780P (2006) https://doi.org/10.1117/12.686232
Event: Optics East 2006, 2006, Boston, Massachusetts, United States

Abstract

The ability to distinguish endospores from each other, from vegetative cells, and from background particles has been demonstrated by PNNL and several other laboratories using various analytical techniques such as MALDI and SIMS. Recent studies at PNNL using Fourier transform Infrared (FTIR) spectroscopy combined with statistical analysis have shown the ability to characterize and discriminate bacterial spores and vegetative bacteria from each other, as well as from background interferents. In some cases it is even possible to determine the taxonomical identity of the species using FTIR. This effort has now grown to include multiple species of bacterial endospores, vegetative cells, and background materials. The present work reports on advances in being able to use FTIR, or IR in combination with other techniques, for rapid and reliable discrimination.

Citation Download Citation

Nancy B. Valentine, Timothy J. Johnson, Yin-Fong Su, and Joel B. Forrester "FTIR spectroscopy for bacterial spore identification and classification", Proc. SPIE 6378, Chemical and Biological Sensors for Industrial and Environmental Monitoring II, 63780P (25 October 2006); https://doi.org/10.1117/12.686232

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