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Synthesis of Infective Poliovirus in BSC-1 Monkey Cells Enucleated with Cytochalasin B

Science
2 Mar 1973
Vol 179, Issue 4076
pp. 915-916

Abstract

BSC-1 monkey kidney cells were enucleated by two cycles of centrifugation in the presence of cytochalasin B. The resulting populations contained 99.5 percent enucleated cells. After infection, newly synthesized poliovirus was recovered from the enucleated populations. Final virus yield per enucleated cell was about one-fifth the yield per infected untreated cell.

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References

CARTER, S.B., EFFECTS OF CYTOCHALASINS ON MAMMALIAN CELLS, NATURE 213: 261 (1967).
CROCKER, T, SCIENCE 145: 40 (1964).
DARNELL, J.E., EARLY EVENTS IN POLIOVIRUS INFECTION, COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY 27: 149 (1962).
FRANKLIN, R.M., PATTERNS OF MACROMOLECULAR SYNTHESIS IN NORMAL AND VIRUS-INFECTED MAMMALIAN CELLS, COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY 27: 175 (1962).
Goldman, R., Locomotion In Tissue Cells (1973).
MARCUS, P.I., METHODS CELL PHYSL 2: 93 (1966).
PRESCOTT, D.M., ENUCLEATION OF MAMMALIAN-CELLS WITH CYTOCHALASIN-B, EXPERIMENTAL CELL RESEARCH 71: 480 (1972).
PRESCOTT, D.M., REPLICATION OF VACCINIA VIRUS DNA IN ENUCLEATED L-CELLS, JOURNAL OF MOLECULAR BIOLOGY 59: 505 (1971).

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Published In

Science
Volume 179 | Issue 4076
2 March 1973

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Published in print: 2 March 1973

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Robert Pollack
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724
Robert Goldman
Department of Biology, Case Western Reserve University, Cleveland, Ohio 44106

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