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Restriction endonuclease Asi256I recognizes and cuts the nucleotide sequence 5′-GATC-3′

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Abstract

A strain producing a restriction endonuclease was isolated from soil samples and identified as the Arthrobacter sp. strain Ck256. The enzyme produced by this strain was termed Asi256I. The isolation procedure for this enzyme was described, and the optimal conditions for its function were determined. It was shown that the restriction endonuclease Asi256I is a true isoschizomer of MboI, it has a temperature optimum of 6°C, and can be used in molecular-biological and genetic-engineering studies performed at low temperatures.

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Authors and Affiliations

  1. Vector State Research Center of Virology and Biotechnology, p. Kol’tsovo, Novosibirsk oblast, 630559, Russia

    T. A. Ushakova, L. I. Puchkova, V. V. Gutorov, O. D. Totmenina & V. E. Repin

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  1. T. A. Ushakova
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  2. L. I. Puchkova
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  3. V. V. Gutorov
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  4. O. D. Totmenina
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  5. V. E. Repin
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Correspondence to V. E. Repin.

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Original Russian Text © T.A. Ushakova, L.I. Puchkova, V.V. Gutorov, O.D. Totmenina, V.E. Repin, 2008, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2008, Vol. 44, No. 1, pp. 34–37.

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Ushakova, T.A., Puchkova, L.I., Gutorov, V.V. et al. Restriction endonuclease Asi256I recognizes and cuts the nucleotide sequence 5′-GATC-3′. Appl Biochem Microbiol 44, 28–31 (2008). https://doi.org/10.1134/S0003683808010043

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  • DOI: https://doi.org/10.1134/S0003683808010043

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