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Inulinases from various producers: The features of their permolecular organization

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Abstract

The structural organization of inulinases from yeasts, fungi, and plants are researched. For studying their sizes, molecular weight, and permolecular organization, an approach consisting of a combination of atomic force microscopy with methods of dynamic light scattering, gel chromatography, and electrophoresis was used. It is shown that inulinases from Kluyveromyces marxianus and Aspergillus niger form geterodimers and inulinases from tubers of Helianthus tuberosus are present as both dimers and monomers. The role of various forms in the functional activity of inulinase molecules is discussed.

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Authors and Affiliations

  1. Voronezh State University, Voronezh, 394006, Russia

    M. G. Holyavka, T. A. Kovaleva, M. V. Grechkina, I. V. Ostankova & V. G. Artyukhov

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  1. M. G. Holyavka
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  2. T. A. Kovaleva
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  3. M. V. Grechkina
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  4. I. V. Ostankova
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  5. V. G. Artyukhov
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Correspondence to M. G. Holyavka.

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Original Russian Text © M.G. Holyavka, T.A. Kovaleva, M.V. Grechkina, I.V. Ostankova, V.G. Artyukhov, 2014, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2014, Vol. 50, No. 1, pp. 17–24.

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Holyavka, M.G., Kovaleva, T.A., Grechkina, M.V. et al. Inulinases from various producers: The features of their permolecular organization. Appl Biochem Microbiol 50, 10–16 (2014). https://doi.org/10.1134/S0003683814010050

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  • DOI: https://doi.org/10.1134/S0003683814010050

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