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Two-dimensional electrophoretic proteome study of serum thermostable fraction from patients with various tumor conditions

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Abstract

One of the problems of plasma proteomics is a presence of large major components. In this work, we use the thermostable fraction as a way to deplete these major proteins. The thermostable fraction of serum samples from patients with ovarian, uterus, and breast cancers and benign ovarian tumor was analyzed using two-dimensional electrophoresis combined with MALDI-TOF(-TOF)-mass spectrometry. Of them, α-1-acid glycoprotein and clusterin are expressly down-regulated in breast cancer, whereas transthyretin is decreased specifically in ovarian cancer. Apolipoprotein A-I forms have decreased spot volumes, while haptoglobin α1, in contrast, is elevated in several tumors. These data are partly consistent with previous art studies on cancer proteomics, which involve mass-spectrometry-based serum profiling techniques. Serum thermostable fraction may be recommended as a good tool for medium and small protein proteome investigation, in particular, by 2D-electrophoresis.

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Abbreviations

2-DE:

two-dimensional electrophoresis

MALDI-TOF:

matrix-assisted laser desorption/ionization time of flight

MS:

mass-spectrometry

SELDI:

surface-enhanced laser desorption/ionization

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Authors and Affiliations

  1. Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, ul. Pogodinskaya 10, 119121, Moscow, Russia

    E. I. Goufman, S. A. Moshkovskii, O. V. Tikhonova, P. G. Lokhov, V. G. Zgoda, M. V. Serebryakova, I. Yu. Toropygin, M. A. Vlasova & A. I. Archakov

  2. Russian State Medical University, ul. Ostrovityanova 1, 117997, Moscow, Russia

    M. R. Safarova & O. V. Makarov

Authors
  1. E. I. Goufman
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  2. S. A. Moshkovskii
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  3. O. V. Tikhonova
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  4. P. G. Lokhov
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  5. V. G. Zgoda
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  6. M. V. Serebryakova
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  7. I. Yu. Toropygin
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  8. M. A. Vlasova
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  9. M. R. Safarova
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  10. O. V. Makarov
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  11. A. I. Archakov
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Corresponding author

Correspondence to S. A. Moshkovskii.

Additional information

Published in Russian in Biokhimiya, 2006, Vol. 71, No. 4, pp. 445–453.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM05-197, February 12, 2006.

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Goufman, E.I., Moshkovskii, S.A., Tikhonova, O.V. et al. Two-dimensional electrophoretic proteome study of serum thermostable fraction from patients with various tumor conditions. Biochemistry (Moscow) 71, 354–360 (2006). https://doi.org/10.1134/S000629790604002X

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  • DOI: https://doi.org/10.1134/S000629790604002X

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