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Structure of a peptidoglycan-related polysaccharide from Providencia alcalifaciens O45

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Abstract

A polysaccharide was isolated from the opportunistic human pathogen Providencia alcalifaciens O45:H26 by extraction with aqueous phenol and studied by sugar and methylation analyses along with 1H and 13C NMR spectroscopy, including two-dimensional ROESY and H-detected 1H,13C HSQC experiments. The polysaccharide contains N-acetylglu-cosamine and N-acetylmuramic acid (D-GlcpNAc3Rlac) amidated with L-alanine and has the following structure:

$$\to 4) - \beta - D - GlcpNAc - (1 \to 4) - \beta - D - GlcpNAc3(Rlac - L - Ala) - (1 \to .$$

The polysaccharide possesses a remarkable structural similarity to the bacterial cell wall peptidoglycan. It is not unique to the strain studied but is common to strains of at least four P. alcalifaciens O-serogroups (O3, O24, O38, and O45). No evidence was obtained that the polysaccharide is associated with the LPS, and hence it might represent a bacterial capsule component.

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Abbreviations

COSY:

correlation spectroscopy

CPS:

capsular polysaccharide

ESI MS:

electrospray ionization mass spectrometry

GlcN:

glucosamine

GlcNAc3Rlac:

N-acetylmuramic acid

HSQC:

heteronuclear single-quantum coherence

LPS:

lipopolysaccharide

Rlac:

(R)-lactic acid or (R)-1-carboxyethyl group

ROESY:

rotating-frame nuclear Overhauser effect spectroscopy

TOCSY:

total correlation spectroscopy

C14:0, C16:0, and C18:0, tetradecanoic, hexadecanoic:

and octadecanoic acid

3OH-C14:0:

3-hydroxytetradecanoic acid

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Authors and Affiliations

  1. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, 119991, Moscow, Russia

    O. G. Ovchinnikova, N. A. Kocharova, A. S. Shashkov, A. N. Kondakova & Y. A. Knirel

  2. TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, 300457, China

    O. G. Ovchinnikova, B. Liu, L. Feng & L. Wang

  3. Department of General Microbiology, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, PL 90-237, Lodz, Poland

    M. Siwinska

  4. Tianjin Key Laboratory of Microbial Functional Genomics, Nankai University, TEDA, Tianjin, 300457, China

    L. Feng & L. Wang

  5. Department of Immunobiology of Bacteria, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, PL 90-237, Lodz, Poland

    A. Rozalski

Authors
  1. O. G. Ovchinnikova
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  2. B. Liu
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  3. N. A. Kocharova
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  4. A. S. Shashkov
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  5. A. N. Kondakova
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  6. M. Siwinska
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  7. L. Feng
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  8. A. Rozalski
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  9. L. Wang
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  10. Y. A. Knirel
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Corresponding author

Correspondence to O. G. Ovchinnikova.

Additional information

Published in Russian in Biokhimiya, 2012, Vol. 77, No. 6, pp. 750–757.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM12-030, March 25, 2012.

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Ovchinnikova, O.G., Liu, B., Kocharova, N.A. et al. Structure of a peptidoglycan-related polysaccharide from Providencia alcalifaciens O45. Biochemistry Moscow 77, 609–615 (2012). https://doi.org/10.1134/S0006297912060077

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  • DOI: https://doi.org/10.1134/S0006297912060077

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