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Application of the method of electro-acoustical analysis for the detection of bacteriophages in a liquid phase

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Abstract

The possibility of the application of electro-acoustic analysis for the detection of bacteriophages was demonstrated for the first time based on the example of the interaction of the FA1-Sp59b bacteriophage with bacterial cells of the strain Azospirillum lipoferum Sp59b. Piezoelectric cross-field resonators with a 1-mL chamber for analyzed liquid were used as the biological sensor. It was revealed that the dependences of the real and imaginary parts of the electrical impedance of the resonator loaded with a suspension of viruses and microbial cells on the frequency was significantly different from those dependences of the resonator that contained a control cell suspension without the virus. It was shown that detection of the FA1-Sp59b bacteriophage using microbial cells was possible with both extraneous viral particles and extraneous microbial cells. The proposed method allows one to accurately determine the type of identified virus after a 5-minute interaction with indicating bacterial culture. As well, the minimum concentration of viruses is five virus particles per cell. These results as a whole demonstrate the possibility of detecting specific interactions of bacteriophages with microbial cells and provide a basis for the development of a biological sensor for the quantitative detection of viruses directly in the liquid phase.

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Authors and Affiliations

  1. Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Enthuziastov 13, Saratov, 410049, Russia

    O. I. Guliy, S. A. Staroverov, L. A. Dykman & O. V. Ignatov

  2. Saratov State Vavilov Agrarian University, Teatralnaya pl. 1, Saratov, 410012, Russia

    O. I. Guliy & S. A. Staroverov

  3. Saratov Scientific and Research Veterinary Institute, Russian Academy of Agricultural Sciences, ul. 53 Strelkovoj Divizii 6, Saratov, 410028, Russia

    O. I. Guliy, S. A. Staroverov & L. A. Dykman

  4. Saratov Branch of the Kotel’nikov Institute of Radio Engineering and Electronics, Russian Academy of Sciences, ul. Zelenaya 38, Saratov, 410019, Russia

    B. D. Zaitsev, A. M. Shikhabudinov & A. A. Teplykh

  5. Kotel’nikov Institute of Radio Engineering and Electronics, Russian Academy of Sciences, ul. Mokhovaya 11/7, Moscow, 125009, Russia

    I. E. Kuznetsova

  6. Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, ul. Akademika Zabolotnogo 154, Kyiv, 03143, Ukraine

    A. B. Balko

  7. Liceum of Natural Sciences, ul. Sovetskaya 60, Saratov, 410056, Russia

    S. S. Makarikhina

Authors
  1. O. I. Guliy
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  2. B. D. Zaitsev
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  3. I. E. Kuznetsova
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  4. A. M. Shikhabudinov
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  5. A. B. Balko
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  6. A. A. Teplykh
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  7. S. A. Staroverov
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  8. L. A. Dykman
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  9. S. S. Makarikhina
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  10. O. V. Ignatov
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Corresponding author

Correspondence to O. I. Guliy.

Additional information

Original Russian Text © O.I. Guliy, B.D. Zaitsev, I.E. Kuznetsova, A.M. Shikhabudinov, A.B. Balko, A.A. Teplykh, S.A. Staroverov, L.A. Dykman, S.S. Makarikhina, O.V. Ignatov, 2016, published in Biofizika, 2016, Vol. 61, No. 1, pp. 60–67.

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Guliy, O.I., Zaitsev, B.D., Kuznetsova, I.E. et al. Application of the method of electro-acoustical analysis for the detection of bacteriophages in a liquid phase. BIOPHYSICS 61, 52–58 (2016). https://doi.org/10.1134/S0006350916010097

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  • DOI: https://doi.org/10.1134/S0006350916010097

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