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Absorption spectra of photosensitized human fat tissue

  • Biomedical Optics and Spectroscopy
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Abstract

We study experimentally the optical characteristics of adipose tissue in vitro in its dye sensitization—by indocyanin green and brilliant green. We conducted experiments on a PerkinElmer Lambda 950 spectrophotometer in the spectral range of 250–900 nm. From an analysis of the measured absorption spectra, we determine the shifts in the maximum of the absorption band for the studied photosensitizers in adipose tissue in comparison to their spectra in solutions. These shifts are explained by the bonding of photosensitizer molecules with the protein matrix of adipose tissue. We find the relative monomer and dimer concentrations of dye molecules in solutions and stained samples.

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Authors and Affiliations

  1. Saratov State Institute, ul. Astrakhanskaya 83, Saratov, 410012, Russia

    I. Yu. Yanina, G. V. Simonenko, V. I. Kochubey & V. V. Tuchin

  2. University of Problems of Precision Mechanics and Control, Russian Academy of Sciences, ul. Rabochaya 24, Saratov, 410028, Russia

    V. V. Tuchin

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  1. I. Yu. Yanina
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  2. G. V. Simonenko
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  3. V. I. Kochubey
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  4. V. V. Tuchin
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Correspondence to I. Yu. Yanina.

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Original Russian Text © I.Yu. Yanina, G.V. Simonenko, V.I. Kochubey, V.V. Tuchin, 2010, published in Optika i Spektroskopiya, 2010, Vol. 109, No. 2, pp. 247–255.

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Yanina, I.Y., Simonenko, G.V., Kochubey, V.I. et al. Absorption spectra of photosensitized human fat tissue. Opt. Spectrosc. 109, 217–224 (2010). https://doi.org/10.1134/S0030400X10080114

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  • DOI: https://doi.org/10.1134/S0030400X10080114

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