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Structure and properties of complexes formed by cationic polymers and anionic cholesterol-containing liposomes

  • Polyelectrolytes
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Abstract

The adsorption of the synthetic polycation poly(N-ethyl-4-vinylpyridinium bromide) on the surface of three-component lipid vesicles (liposomes) formed from a mixture of anionic cardiolipin, electroneutral egg lecithin, and nonionic cholesterol is studied via laser microelectropheresis, dynamic light scattering, conductometry, fluorescence spectroscopy, and UV spectroscopy. The incorporation of cholesterol into the liposomal membrane increases its microviscosity; however, the membrane remains liquid-crystalline. Simultaneously, an increase in the fraction of cholesterol causes the formation of defects in liposome membranes during their binding with poly(N-ethyl-4-vinylpyridium bromide) and makes complexation irreversible. The results of this study are of interest for predicting the behavior of polyelectrolytes and biologically active structures formed on their basis on the surface of cells and the reaction of the cellular membrane to the adsorbed polymer.

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Authors and Affiliations

  1. Faculty of Chemistry, Moscow State University, Moscow, 119991, Russia

    A. A. Yaroslavov, A. A. Efimova & S. N. Kostenko

Authors
  1. A. A. Yaroslavov
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  2. A. A. Efimova
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  3. S. N. Kostenko
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Correspondence to A. A. Efimova.

Additional information

Original Russian Text © A.A. Yaroslavov, A.A. Efimova, S.N. Kostenko, 2012, published in Vysokomolekulyarnye Soedineniya, Ser. A, 2012, Vol. 54, No. 4, pp. 541–547.

This work was supported by the Russian Foundation for Basic Research, project nos. 11-03-00936 and 11-03-92487.

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Yaroslavov, A.A., Efimova, A.A. & Kostenko, S.N. Structure and properties of complexes formed by cationic polymers and anionic cholesterol-containing liposomes. Polym. Sci. Ser. A 54, 264–269 (2012). https://doi.org/10.1134/S0965545X12040086

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  • DOI: https://doi.org/10.1134/S0965545X12040086

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