Skip to main content
SpringerLink
Log in

Interactive effect of nitric oxide and brassinosteroids on photosynthesis and the antioxidant system of Lycopersicon esculentum

  • Research Papers
  • Published:
Russian Journal of Plant Physiology Aims and scope Submit manuscript

Abstract

An interactive effect of nitric oxide and 24-epibrassinolide (EBL) or 28-homobrassinolide (HBL) was investigated in Lycopersicon esculentum Mill. cv. K-21. Seeds prior to sowing were exposed to sodium nitroprusside (SNP) (0, 10−5 M or 1 M), a nitric oxide donor, for 8 h, and the resulting seedlings were subsequently sprayed with 10−8 M EBL or HBL at 30-day stage. The analysis of plant samples after 24 h of the EBL or HBL spray showed that the lower SNP concentration (10−5 M) favored the growth, pigment content, photosynthetic and enzymatic activities, and also improved the antioxidant system. However, 1 M SNP had inhibitory impact on most of the indices, except the antioxidant system. Brassinosteroids (EBL or HBL), on the other hand, had a stimulatory impact on the aforementioned indices. The inhibitory effect of SNP (1 M) was neutralized by the application of BRs, where EBL was more effective than HBL.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BR:

brassinosteroid

CA:

carbonic anhydrase

EBL:

24-epibrassinolide

HBL:

28-homobrassinolide

MSI:

membrane stability index

NR:

nitrate reductase

RWC:

relative water content

SNP:

sodium nitroprusside

TBARS:

thiobarbituric acid reactive substances

References

  1. Leshem, Y.Y., Nitric Oxide in Plants: Occurrence, Function and Use, Dordrecht: Kluwer, 2000.

    Google Scholar 

  2. Guo, F., Okamoto, M., and Crawford, N.M., Identification of a Plant Nitric Oxide Synthase Gene Involved in Hormonal Signaling, Science, 2003, vol. 302, pp. 100–103.

    Article  CAS  PubMed  Google Scholar 

  3. Neill, S.J., Desikan, R., and Hancock, J.T., Nitric Oxide Signaling in Plants, New Phytol., 2003, vol. 159, pp. 11–35.

    Article  CAS  Google Scholar 

  4. Arasimowicz, M. and Floryszak-Wieczorek, J., Nitric Oxide as a Bioactive Signaling Molecule in Plant Stress Responses, Plant Sci., 2007, vol. 172, pp. 876–887.

    Article  CAS  Google Scholar 

  5. Stoehr, C. and Ulrich, W.R., Generation and Possible Roles of NO in Plant Roots and Their Apoplastic Space, J. Exp. Bot., 2002, vol. 53, pp. 2293–2303.

    Article  Google Scholar 

  6. Kramlinch, J.C. and Linak, W.P., Nitrous Oxide Behavior in the Atmosphere and in Combustion and Industrial Systems, Prog. Energy Combus. Sci., 1994, vol. 20, pp. 149–202.

    Article  Google Scholar 

  7. Sasse, J.M., Physiological Actions of Brassinosteroids: An Update, J. Plant Growth Regul., 2003, vol. 22, pp. 276–288.

    Article  CAS  PubMed  Google Scholar 

  8. Ali, B., Hayat, S., and Ahmad, A., 28-Homobrassinolide Ameliorates the Saline Stress in Chickpea (Cicer arietinum L.), Environ. Exp. Bot., 2007, vol. 59, pp. 217–223.

    Article  CAS  Google Scholar 

  9. Hasan, S.A., Hayat, S., Ali, B., and Ahmad, A., 28-Homobrassinolide Protects Chickpea (Cicer arietinum) from Cadmium Toxicity by Stimulating Antioxidants, Environ. Pollut., 2008, vol. 151, pp. 60–66.

    Article  CAS  PubMed  Google Scholar 

  10. Lichtenthaler, H.K., Chlorophylls and Carotenoids: Pigments of Photosynthetic Biomembranes, Methods Enzymol., 1987, vol. 148, pp. 350–382.

    Article  CAS  Google Scholar 

  11. Hodges, M.D., DeLong, J.M., Forney, C.F., and Prange, R.K., Improving the Thiobarbituric Acid Reactive Substances Assay for Lipid Peroxidation in Plant Tissues Containing Anthocyanin and Other Interfering Compounds, Planta, 1999, vol. 207, pp. 604–611.

    Article  CAS  Google Scholar 

  12. Terasaki, S., Sakurai, N., Yamamoto, R., Wada, N., and Nevins, D.J., Changes in Cell Wall Polysaccharides of Kiwifruit and the Viscoelastic Properties Detected by Laser Doppler Method, J. Jap. Soc. Hortic. Sci., 2001, vol. 70, pp. 572–580.

    Article  CAS  Google Scholar 

  13. Zhang, M., An, L., Feng, H., Chen, T., Chen, K., Liu, Y., Tang, H., Chang, J., and Wang, X., The Cascade Mechanisms of Nitric Oxide as a Second Messenger of Ultraviolet-B in Inhibiting Mesocotyl Elongations, Photochem. Photobiol., 2003, vol. 77, pp. 219–225.

    Article  CAS  PubMed  Google Scholar 

  14. Beligni, M.V. and Lamattina, L., Nitric Oxide Stimulates Seed Germination and De-Etiolation and Inhibits Hypocotyls Elongation, Three Light Inducible Responses in Plants, Planta, 2000, vol. 210, pp. 215–221.

    Article  CAS  PubMed  Google Scholar 

  15. Felner, M., Recent Progress in Brassinosteroid Research: Hormone Perception and Signal Transduction, Brassinosteroids: Bioactivity and Crop Productivity, Hayat, S. and Ahmad, A., Eds., Dordrecht: Kluwer, 2003, pp. 69–86.

    Google Scholar 

  16. Cosgrove, D., Relaxation in a High Stress Environment: The Molecular Basis of Extensible Cell Walls and Enlargement, Plant Cell, 1997, vol. 9, pp. 1031–1041.

    Article  CAS  PubMed  Google Scholar 

  17. Ali, B., Hayat, S., Hasan, S.A., and Ahmad, A., Effect of Root Applied 28-Homobrassinolide on the Performance of Lycopersicon esculentum, Sci. Hortic., 2006, vol. 110, pp. 267–273.

    Article  CAS  Google Scholar 

  18. Garcia-Mata, C. and Lamattina, L., Nitric Oxide Induces Stomatal Closure and Enhances the Adaptive Plant Responses against Drought Stress, Plant Physiol., 2001, vol. 126, pp. 1196–1204.

    Article  CAS  PubMed  Google Scholar 

  19. Tiwari, A., Kumar, P., Singh, S., and Ansari, S.A., Carbonic Anhydrase in Relation to Higher Plants, Photosynthetica, 2005, vol. 43, pp. 1–9.

    Article  CAS  Google Scholar 

  20. Yu, J.Q., Huang, L.F., Hu, W.H., Zhou, Y.H., Mao, W.H., Ye, S.F., and Nogues, S., A Role of Brassinosteroids in the Regulation of Photosynthesis in Cucumis sativus, J. Exp. Bot., 2004, vol. 55, pp. 1135–1143.

    Article  CAS  PubMed  Google Scholar 

  21. Beligni, M.V. and Lamattina, L., Nitric Oxide Counteracts Cytotoxic Processes Mediated by Reactive Oxygen Species in Plant Tissue, Planta, 1999, vol. 208, pp. 337–344.

    Article  CAS  Google Scholar 

  22. Campbell, H.W., Nitrate Reductase Structure, Function and Regulation. Bridging the Gap between Biochemistry and Physiology, Annu. Rev. Plant Physiol. Plant Mol. Biol., 1999, vol. 50, pp. 277–303.

    Article  CAS  PubMed  Google Scholar 

  23. Kulaeva, O.N., Burakhanova, E.A., Fedina, A.B., Khokhlova, V.A., Bokebayeva, G.A., Vorbrodt, H.M., and Adam, G., Effect of Brassinosteroid on Protein Synthesis and Plant Cell Ultrastructure under Stress Condition, ACS Symp., Ser. 474, Brassinosteroids: Chemistry, Bioactivity and Applications, Cutler, H.G., Yokata, T., and Adam, G., Eds., Washington: Am. Chem. Soc., 1999, pp. 141–155.

    Google Scholar 

  24. Dawson, T.M., Nitric Oxide, PARP and Other Perpetrators Relevant to Stroke and Neurodegeneration, Progress in Brain Research, 1998, http://www.pas-teur.fr/conf/nitricabstracts.html.

  25. Kehrer, J., Free Radicals as Mediators of Tissue Injury and Diseases, Crit. Rev. Toxicol., 1993, vol. 23, pp. 21–48.

    Article  CAS  PubMed  Google Scholar 

  26. Kanner, J., Harel, S., and Granit, R., Nitric Oxide as an Antioxidant, Arch. Biochem. Biophys., 1991, vol. 289, pp. 130–136.

    Article  CAS  PubMed  Google Scholar 

  27. Joshi, M.S., Ponthier, J.L., and Lancaster, J.R., Cellular Antioxidant and Prooxidant Actions of Nitric Oxide, Free Radic. Biol. Med., 1999, vol. 27, pp. 1357–1366.

    Article  CAS  PubMed  Google Scholar 

  28. Lamattina, L., Garcia-Mata, C., Graziano, M., and Pagnussat, G., Nitric Oxide: The Versatility of an Extensive Signal Molecule, Annu. Rev. Plant Biol., 2003, vol. 54, pp. 109–136.

    Article  CAS  PubMed  Google Scholar 

  29. Halliwell, B. and Gutteridge, J.M.C., Free Radicals in Biology and Medicine, London: Oxford Univ. Press, 1999.

    Google Scholar 

  30. Cao, S., Xu, Q., Cao, Y., Qian, K., An, K., Zhu, Y., Binzeng, H., Zhao, H., and Kuai, B., Loss of Function Mutations in Det2 Gene Lead to an Enhanced Resistance to Oxidative Stress in Arabidopsis, Physiol. Plant., 2005, vol. 123, pp. 57–66.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Plant Physiology Section, Department of Botany, Aligarh Muslim University, Aligarh, 202002, India

    Sh. Hayat & S. Yadav

  2. Department of Botany, Government College for Women, M.A. Road, Srinagar, Kashmir, India

    B. Ali

  3. Department of Applied Sciences, Higher College of Technology, Al-Khuwair, Sultanate of Oman

    A. Ahmad

Authors
  1. Sh. Hayat
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Yadav
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. Ali
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Ahmad
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sh. Hayat.

Additional information

Published in Russian in Fiziologiya Rastenii, 2010, Vol. 57, No.2, pp. 224–233.

This text was submitted by the authors in English.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hayat, S., Yadav, S., Ali, B. et al. Interactive effect of nitric oxide and brassinosteroids on photosynthesis and the antioxidant system of Lycopersicon esculentum . Russ J Plant Physiol 57, 212–221 (2010). https://doi.org/10.1134/S1021443710020081

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1021443710020081

Key words

Search

Navigation