Abstract
Based on modern technologies of molecular DNA-markers, blast disease–resistance genes (Pi-ta, Pi-b, Pi-1, Pi-2, and Pi-33) were introgressed and pyramided into domestic rice varieties to give them longterm disease resistance. For that purpose, this case study uses SSR-markers closely linked to these genes, as well as intragenic markers of genes Pi-ta and Pi-b. Multiplex PCR systems were created for simultaneous identification of two resistance genes in the hybrid progeny for the following combinations: Pi-1 + Pi-2, Pi-ta + Pi-b, Pi-ta + Pi-33.
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Original Russian Text © E.V. Dubina, Zh.M. Mukhina, E.M. Kharitonov, V.N. Shilovskiy, E.S. Kharchenko, L.V. Esaulova, N.N. Korkina, E.P. Maximenko, I.B. Nikitina, 2015, published in Genetika, 2015, Vol. 51, No. 8, pp. 881–886.
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Dubina, E.V., Mukhina, Z.M., Kharitonov, E.M. et al. Creation of blast disease-resistant rice varieties with modern DNA-markers. Russ J Genet 51, 752–756 (2015). https://doi.org/10.1134/S1022795415060058
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DOI: https://doi.org/10.1134/S1022795415060058