Skip to main content
SpringerLink
Log in

Determination of glimepiride in pharmaceutical formulations using HPLC and first-derivative spectrophotometric methods

  • Articles
  • Published:
Journal of Analytical Chemistry Aims and scope Submit manuscript

Abstract

Two validated analytical methods have been developed to determine glimepiride in pharmaceutical formulations using HPLC and 1st order derivative spectrophotometric techniques. Employing reverse phase HPLC method, the drug was analyzed by pumping a mixture of acetonitrile and 2% formic acid solution, pH 3.5 (80: 20 v/v) through a C18 column (250 × 4.6 mm, 5 μm) and detecting the eluents at 228 nm. The linearity range was found to be 20–140 μg/mL with mean recovery of 100.52 ± 0.33%. The second method was based on the formation of a complex of the drug with 2,3,5-triphenyl-2H-tetrazolium chloride in basic media. 1st order derivative spectrum made it possible to detect the complex at 413.5 nm. The linearity range was found to be 40–160 μg/mL, with mean recovery of 100.33 ± 0.47%. Both the proposed methods can reliably be used for routine analysis of glimepiride in raw material as well as in pharmaceutical formulations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Maryadele, J. O’Neil, Merck Index, Whitehouse Station, NJ: Merck, 2001.

    Google Scholar 

  2. Drager, E., Diab. Res. Clin. Pract., 1995, vol. 28, p. 139.

    Article  Google Scholar 

  3. Pistos, C., Koutsopoulou, M., and Panderi, I., Biomed. Chromatogr., 2005, vol. 19, no. 5, p. 394.

    Article  CAS  Google Scholar 

  4. Salem, I.I., Idrees, J., and Al Tamimi, J.I., J. Chromatogr., B, 2004, vol. 799, no. 1, p. 103.

    Article  CAS  Google Scholar 

  5. Kim, H., Chang, K.Y., Park, C.H., Jang, M.S., Lee, J., Lee, H.J., and Lee, K.R., Chromatographia, 2004, vol. 60, nos. 1–2, p. 93.

    CAS  Google Scholar 

  6. Lehr, K.H. and Damm, P., J. Chromatogr., 1990, vol. 526, no. 2, p. 497.

    CAS  Google Scholar 

  7. Khan, M.A., Sinha, S., Vartak, S., Bhartiya, A., and Kumar, S., J. Pharm. Biomed. Anal., 2005, vol. 39, no. 5, p. 928.

    Article  CAS  Google Scholar 

  8. El Deeb, S., Schepers, U., and Watzig, H., J. Sep. Sci., 2006, vol. 29, no. 11, p. 1571.

    Article  Google Scholar 

  9. Altinoz, S. and Tekeli, D., J. Pharm. Biomed. Anal., 2001, vol. 4, no. 3, p. 507.

    Article  Google Scholar 

  10. Kovarikova, P., Klimes, J., Dohnal, J., and Tisovska, L., J. Pharm. Biomed. Anal., 2004, vol. 36, p. 205.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Government College University, Lahore, 54000, Pakistan

    I. U. Khan, F. Aslam, M. Ashfaq & M. N. Asghar

Authors
  1. I. U. Khan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. Aslam
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Ashfaq
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. N. Asghar
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to I. U. Khan.

Additional information

The article is published in the original.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Khan, I.U., Aslam, F., Ashfaq, M. et al. Determination of glimepiride in pharmaceutical formulations using HPLC and first-derivative spectrophotometric methods. J Anal Chem 64, 171–175 (2009). https://doi.org/10.1134/S1061934809020130

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1061934809020130

Keywords

Search

Navigation