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Analysis of the Mutual Influence of the Microalgae Heterosigma akashiwo (Raphidophyceae) and Thalassiosira pseudonana (Bacillariophyta)

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Abstract

The mutual influence of the rafidophyte microalgae Heterosigma akashiwo, which causes algal blooms, and the diatom Thalassiosira pseudonana, which is regularly present in sea waters, was studied under experimental conditions. Experiments were carried out with monocultures, monocultural filtrates, and mixed cultures of different initial concentrations. The growth and physiological state of the microalgae were assessed by flow cytometry. Both algae displayed inhibited growth in co-culture and on filtrates, but growth was suppressed to a greater extent in T. pseudonana. The fluorescence of chlorophyll a and the contents of reactive oxygen species and neutral lipids decreased in H. akashiwo cultures grown on T. pseudonana filtrate. Similar changes were noted in a T. pseudonana culture grown on H. akashiwo filtrate. It is concluded that these algae exhibit allelopathic activity in relation to each other.

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ACKNOWLEDGMENTS

Microalgae cultures Heterosigma akashiwo (Raphidophyceae) MBRU_HAK-SR11 and Thalassiosira pseudonana MBRU_TSP-02 (Bacillariophyta) were provided by the Marine Biobank Resource Center of the NSCMB FEB RAS (http://marbank.dvo.ru).

Funding

This work was supported by a grant from the Russian Science Foundation (project no. 21-74-30004).

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  1. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, 690041, Vladivostok, Russia

    Zh. V. Markina

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Correspondence to Zh. V. Markina.

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Translated by I. Goll

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Markina, Z.V. Analysis of the Mutual Influence of the Microalgae Heterosigma akashiwo (Raphidophyceae) and Thalassiosira pseudonana (Bacillariophyta). Russ J Mar Biol 48, 353–361 (2022). https://doi.org/10.1134/S1063074022050170

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