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Variation at Nuclear Loci in the Japanese Sea Cucumber Apostichopus japonicus (Selenka, 1867) (Echinodermata: Holothuroidea) in Samples from Peter the Great Bay, Sea of Japan

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Abstract

The genetic diversity of the sea cucumber Apostichopus japonicus was assessed using five microsatellite markers. A total of 122 specimens of sea cucumber from five samples collected in Peter the Great Bay (Sea of Japan) were examined. All loci were polymorphic. For all samples and loci, the average value of the observed heterozygosity was 0.461 ± 0.027 and the expected heterozygosity was 0.575 ± 0.031. The values of the inbreeding coefficient were, on average, greater than zero due to the deficiency of heterozygotes. Null alleles were identified for some microsatellite loci. After their detection, genotyping errors and statistical data were corrected. The presence of null alleles caused a 1.5–2-fold decrease in the value of observed heterozygosity and led to deviations from the Hardy–Weinberg equilibrium.

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ACKNOWLEDGMENTS

The authors are grateful to E.I. Bondar (Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences), I. Chybicki (Casimir the Great University, Poland), and M.J. Dąbrowski (Museum and Institute of Zoology of the Polish Academy of Sciences) for help in research.

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  1. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, 690041, Vladivostok, Russia

    V. D. Yagodina, N. M. Batishcheva & V. A. Brykov

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  1. V. D. Yagodina
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Translated by T. Koznova

APPENDIX

APPENDIX

Таблица 6. Null allele frequency for 5 loci of the sea cucumber samples according to statistical programs
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Yagodina, V.D., Batishcheva, N.M. & Brykov, V.A. Variation at Nuclear Loci in the Japanese Sea Cucumber Apostichopus japonicus (Selenka, 1867) (Echinodermata: Holothuroidea) in Samples from Peter the Great Bay, Sea of Japan. Russ J Mar Biol 48, 380–388 (2022). https://doi.org/10.1134/S1063074022050212

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