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The effects of alpha-tocoferol and H2O2 on the mitochondrial membrane potential and Bax/Bcl-xL ratio in PC12 cells

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Abstract

Using the flow-cytometry method, we demonstrated the ability of alpha-tocopherol (alpha-T) at nano- and micromolar concentrations to recover the mitochondrial membrane potential (ΔΨm), which decreased after H2O2 treatment in PC12 neuronal line cells. Alterations in the ΔΨm in PC12 cells were evaluated using the fluorescent probe tetramethylrhodamine (TMRM). The higher cell-fluorescence intensity corresponded to a higher ΔΨm value. Treatment with H2O2 resulted in a decrease in the fluorescence intensity from 100% in the control to 43.2 ± 5.1% (p < 0.001). Long-term 18-h preincubation with 100 nM or 100 μM alpha-T increased the fluorescence intensity in PC12 cells from 43.2 ± 5.2% up to 88.4 ± 4.8 and 89.6 ± 6.5% of the control value, respectively (p < 0.01 according to the one-way analysis of variances). Alpha-T at both 100 nM and 100 μM concentrations significantly and with similar efficacy decreased the ratio of the proapoptotic protein Bax to the antiapoptotic protein Bcl-xL (Bax/Bcl-xL) in the control PC12 cells. However, we did not reveal any significant effect of H2O2 or preincubation with alpha-T on this ratio in PC12 lysates. Furthermore, H2O2 increased both the Bax and Bcl-xL levels in PC12 cells.

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Authors and Affiliations

  1. Sechenov Institution of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia

    Yu. A. Vlasova, I. O. Zakharova & N. F. Avrova

  2. pr. Toreza, 37, St. Petersburg, 194223, Russia

    N. F. Avrova

Authors
  1. Yu. A. Vlasova
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  2. I. O. Zakharova
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  3. N. F. Avrova
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Correspondence to N. F. Avrova.

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Original Russian Text © Yu.A. Vlasova, I.O. Zakharova, N.F. Avrova, 2016, published in Neirokhimiya, 2016, Vol. 33, No. 4, pp. 344᾿49.

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Vlasova, Y.A., Zakharova, I.O. & Avrova, N.F. The effects of alpha-tocoferol and H2O2 on the mitochondrial membrane potential and Bax/Bcl-xL ratio in PC12 cells. Neurochem. J. 10, 318–322 (2016). https://doi.org/10.1134/S1819712416040152

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  • DOI: https://doi.org/10.1134/S1819712416040152

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