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Single-Cell Force Spectroscopy of Interaction of Lipopolysaccharides from Yersinia pseudotuberculosis and Yersinia pestis with J774 Macrophage Membrane Using Optical Tweezers

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Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

In order to investigate quantitatively the role of lipopolysaccharides (LPS) from outer bacterial membrane at the initial state of bacterium adhesion to a host cell membrane, a model system for single cell force spectroscopy was developed and used. The system comprised of an LPS-coated microsphere placed into optical trap and a J774 macrophage being approached the microsphere to initiate their binding and then moved back to rupture the bond. An “object shadow” phenomenon was discovered, manifested as large-scale variations of the signal of photodetector registering the trapped microsphere displacement, such variations emerging long before the actual interaction between the macrophage and microsphere. The theory and the measurements technique were developed for registration of the force required for detachment of bounded microsphere from the object investigated by means of optical tweezers under the “object shadow” conditions. Characteristic spectra of binding force between J774 macrophage and microspheres functionalized with various LPS, as well as LPS plus complementary antibodies preparations were obtained at the rate of detachment force application of 3–6 pN/s. Force spectrum characteristic of Yersinia pseudotuberculosis LPS possessing O-antigen had a maximum at ~14 pN with half-width of ~23 pN. The treatment of O-antigen with complementary antibodies resulted in transformation of this spectrum into a spectrum with maximum at ~10 pN and half-width of ~14 pN, being almost identical to the spectrum of Y. pestis LPS devoid of O-antigen, with a maximum at ~9 pN and half-width of ~13 pN. A possible mechanism of force spectra formation has been proposed under assumptions of nonspecific binding of O-antigen and probable receptor-type binding of LPS core region to the macrophage surface. The elastic modulus of macrophage envelope, as estimated using analysis of displacement of the contacting microsphere as an indenter, was ≈0.17 pN/nm.

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Authors and Affiliations

  1. Vyatka State University, Kirov, 610000, Russia

    A. A. Byvalov & I. V. Konyshev

  2. Institute of Physiology, Komi Research Center, Ural Branch, Russian Academy of Sciences, Syktyvkar, 167982, Russia

    A. A. Byvalov & I. V. Konyshev

  3. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, 119334, Russia

    V. L. Kononenko

  4. Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow, 117997, Russia

    V. L. Kononenko

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  1. A. A. Byvalov
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  2. V. L. Kononenko
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  3. I. V. Konyshev
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Correspondence to V. L. Kononenko.

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Original Russian Text © A.A. Byvalov, V.L. Kononenko, I.V. Konyshev, 2018, published in Biologicheskie Membrany, 2018, Vol. 35, No. 2, pp. 115–130.

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Byvalov, A.A., Kononenko, V.L. & Konyshev, I.V. Single-Cell Force Spectroscopy of Interaction of Lipopolysaccharides from Yersinia pseudotuberculosis and Yersinia pestis with J774 Macrophage Membrane Using Optical Tweezers. Biochem. Moscow Suppl. Ser. A 12, 93–106 (2018). https://doi.org/10.1134/S1990747818020058

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