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The status of the macromolecular crystallography beamlines at the European Synchrotron Radiation Facility

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Abstract

The European Synchrotron Radiation Facility (ESRF) is the oldest and most powerful 3rd generation synchrotron in Europe, providing X-rays to more than 40 experimental stations welcoming several thousand researchers per year. A major success story has been the ESRF's facilities for macromolecular crystallography (MX). These are grouped around 3 straight sections: On ID23 canted undulators accommodate ID23-1, a mini-focus tuneable energy end station and ID23-2, the world's first micro-focus beamline dedicated to MX; ID29 houses a single, mini-focus, tuneable energy end station; ID30 will provide three end stations for MX due in operation from mid-2014 to early 2015. Here, one branch of a canted X-ray source feeds two fixed-energy end stations (MASSIF-1, MASSIF-3). The second feeds ID30B, a variable focus, tuneable energy beamline. MASSIF-1 is optimised for automatic high-throughput experiments requiring a relatively large beam size at the sample position, MASSIF-3 is a high-intensity, micro-focus facility designed to complement ID23-2. All end stations are highly automated, equipped with sample mounting robots and large area, fast-readout photon-counting detectors. Experiment control and tracking is achieved via a combination of the MXCuBE2 graphical user interface and the ISPyB database, the former allowing user-friendly control of all beamline components, the latter providing data tracking before, after and during experiments.

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Author information

Authors and Affiliations

  1. European Synchrotron Radiation Facility, Structural Biology Group, CS 40220, 38043, Grenoble Cedex 9, France

    Christoph Mueller-Dieckmann, Philippe Carpentier, David Flot, Didier Nurizzo, Petra Pernot, Alexander Popov, Antoine Royant, Daniele de Sanctis, David von Stetten & Gordon A. Leonard

  2. EMBL Grenoble Outstation, 71 avenue des Martyrs, CS 90181, 38042, Grenoble Cedex 9, France

    Matthew W. Bowler, Andrew A. McCarthy, Max H. Nanao & Adam Round

  3. Unit for Virus Host-Cell Interactions, University of Grenoble Alpes-EMBL-CNRS, 71 Avenue des Martyrs, 38042, Grenoble Cedex 9, France

    Matthew W. Bowler, Andrew A. McCarthy, Max H. Nanao & Adam Round

  4. Institut de Biologie Structurale, 71 avenue des Martyrs, CS 10090, 38044, Grenoble Cedex 9, France

    Antoine Royant

Authors
  1. Christoph Mueller-Dieckmann
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  2. Matthew W. Bowler
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  3. Philippe Carpentier
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  4. David Flot
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  5. Andrew A. McCarthy
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  6. Max H. Nanao
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  7. Didier Nurizzo
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  8. Petra Pernot
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  9. Alexander Popov
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  10. Adam Round
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  11. Antoine Royant
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  12. Daniele de Sanctis
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  13. David von Stetten
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  14. Gordon A. Leonard
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Corresponding author

Correspondence to Gordon A. Leonard.

Additional information

Contribution to the Focus Point on “Status of third-generation synchrotron crystallography beamlines: An overview” edited by Gaston Garcia.

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Mueller-Dieckmann, C., Bowler, M.W., Carpentier, P. et al. The status of the macromolecular crystallography beamlines at the European Synchrotron Radiation Facility. Eur. Phys. J. Plus 130, 70 (2015). https://doi.org/10.1140/epjp/i2015-15070-0

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  • DOI: https://doi.org/10.1140/epjp/i2015-15070-0

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