Abstract
The localization of vanilloid receptor TRPV1 was studied in rat gastric fundus by an immunohistochemical technique. Numerous TRPV1-immunoreactive nerve fibers were found around arterioles in the submucosal layer. A large number of the nerve fibers were also seen in the smooth muscle layer and in the myenteric nerve plexus, but the cell bodies could not be found. TRPV1 nerve fibers within the circular muscle layers were running parallel to the muscle fibers. Virtually all TRPV1 axons were immunoreactive for calcitonin-gene-related peptide (CGRP), with particularly extensive double labeling seen in axons of the submucosa around blood vessels. TRPV1 nerve fibers containing substance P were found running in longitudinal muscle and circular muscle. The TRPV1 axons seem to be predominantly extrinsic and contain CGRP and substance P in gastric fundus. TRPV1 neurons are thought to be sensory afferent neurons that operate to maintain gastric motility and blood flow.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anavi-Goffer, S. and Coutts, A. A. (2003). Cellular distribution of vanilloid VR1 receptor immunoreactivity in the guinea-pig myenteric plexus, Eur. J. Pharmacol. 458, 61–71.
Bartho, L., Benko, R., Patacchini, R., et al. (2004). Effects of capsaicin on visceral smooth muscle: a valuable tool for sensory neurotransmitter identification, Eur. J. Pharmacol. 500, 143–157.
Caterina, M. J., Schumacher, M. A., Tominaga, M., et al. (1997). The capsaicin receptor: a heatactivated ion channel in the pain pathway, Nature 389, 816–824.
Gates, T. S., Zimmerman, R. P., Manthyh, C. R., et al. (1989). Calcitonin gene-related peptide-alpha receptor binding sites in the gastrointestinal tract, Neuroscience 31, 757–770.
Green, T. and Dockray, G. J. (1988). Characterization of the peptidergic afferent innervation of the stomach in the rat, mouse and guinea-pig, Neuroscience 25, 181–193.
Holzer, P. (1998). Neural emergency system in the stomach, Gastroenterology 114, 823–839.
Holzer, P. and Holzer-Petsche, U. (2001). Tachykinin receptors in the gut: physiological and pathological implications, Curr. Opin. Pharmacol. 1, 583–590.
Holzer-Petsche, U., Seitz, H. and Lembeck, F. (1989). Effect of capsaicin on gastric corpus smooth muscle of the rat in vitro, Eur. J. Pharmacol. 162, 29–36.
Horie, S., Yamamoto, H., Michael, G. J., et al. (2004). Protective role of vanilloid receptor type 1 in HCl-induced gastric mucosal lesions in rats, Scand. J. Gastroenterol. 39, 303–312.
Kadowaki, M., Kuramoto, H. and Takaki, M. (2004). Combined determination with functional and morphological studies of origin of nerve fibers expressing transient receptor potential vanilloid 1 in the myenteric plexus of the rat jejunum, Auton. Neurosci. 116, 11–18.
Kato, S., Aihara, E., Nakamura, A., et al. (2003). Expression of vanilloid receptors in rat gastric epithelial cells: role in cellular protection, Biochem. Pharmacol. 66, 1115–1121.
Michael, G. J. and Priestley, J. V. (1999). Differential expression of the mRNA for the vanilloid receptor subtype 1 in cells of the adult rat dorsal root and nodose ganglia and its downregulation by axotomy, J. Neurosci. 19, 1844–1854.
Mózski, G., Abdel-Salam, O. M. E. and Szolcányi, J. (Eds) (1997). Experimental gastric ulcer studies, in: Capsaicin-sensitive Afferent Nerves in Gastric Mucosal Damage and Protection, pp. 41–94. Akadémiai Kiadó, Budapest.
Nozawa, Y., Nishihara, K., Yamamoto, A., et al. (2001). Distribution and characterization of vanilloid receptors in the stomach, Neurosci. Lett. 309, 33–36.
Patterson, L. M., Zheng, H., Ward, S. M., et al. (2003). Vanilloid receptor (VR1) expression in vagal afferent neurons innervating the gastrointestinal tract, Cell Tissue Res. 311, 277–287.
Poonyachoti, S., Kulkarni-Narla, A. and Brown, D. R. (2002). Chemical coding of neurons expressing delta-and kappa-opioid receptor and type I vanilloid receptor immunoreactivities in the porcine ileum, Cell Tissue Res. 307, 23–33.
Schicho, R., Florian, W., Liebmann, I., et al. (2004). Increased expression of TRPV1 receptor in dorsal root ganglia by acid insult of the rat gastric mucosa, Eur. J. Neurosci. 19, 1811–1818.
Schmidt, P. T. and Holst, J. J. (2000). Tachykinins in regulation of gastric motility and secretion, Cell. Mol. Life Sci. 57, 579–588.
Sternini, C., Reeve Jr., J. R. and Brecha, N. (1987). Distribution and characterization of calcitonin gene-related peptide immunoreactivity in the digestive system of normal and capasaicin-treated rats, Gastroenterology 93, 852–862.
Takeuchi, K., Ueshima, K., Ohuchi, T., et al. (1994). The role of capsaicin-sensitive sensory neurons in healing of HCl-induced gastric mucosal lesions in rats, Gastroenterology 106, 1524–1532.
Tominaga, M., Caterina, M. J., Malmberg, A. B., et al. (1998). The cloned capsaicin receptor integrates multiple pain-producing stimuli, Neuron 21, 531–543.
Ward, S. M., Bayguinov, J., Won, K. J., et al. (2003). Distribution of the vanilloid receptor (VR1) in the gastrointestinal tract, J. Comp. Neurol. 465, 121–135.
Yamamoto, H., Horie, S., Uchida, M., et al. (2001). Effects of vanilloid receptor agonists and antagonists on gastric antral ulcers in rats, Eur. J. Pharmacol. 432, 203–210.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Horie, S., Michael, G.J. & Priestley, J.V. Co-localization of TRPV1-expressing nerve fibers with calcitonin-gene-related peptide and substance P in fundus of rat stomach. Inflammopharmacol 13, 127–137 (2005). https://doi.org/10.1163/156856005774423854
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1163/156856005774423854