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Different Expression of Hypoxic and Angiogenic Factors in Human Endometriotic Lesions

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Abstract

Endometriosis is associated with local angiogenic and hypoxic mechanisms. Indeed, peritoneal fluid of women with endometriosis generates a specific microenvironment to support the growth and development of ectopic endometrial tissues. The association between proangiogenic markers and hypoxic processes in different endometriosis phenotypes was investigated in the present study, analyzing the expression of several genes, related to hypoxic signaling pathway and involved in angiogenic processes, in nonpregnant women with different forms of endometriosis. Samples of ovarian endometrioma (OMA; n = 16) or deep infiltrating endometriosis (DIE; n = 11) were collected, and in addition, control endometrium was collected from healthy women by hysteroscopy. The gene expression of the hypoxia-inducible factors (HIF) 1/2α, protease-activated receptors (PARs) ¼, and vascular endothelial growth factor (VEGF) A was evaluated by quantitative reverse-transcription polymerase chain reaction. Ovarian endometrioma expresses high levels of HIF-1/2α, PAR-1/4, and VEGF-A, while DIE did not show significantly different gene expression compared to endometrium from unaffected women. A positive correlation between the expression of HIF-1/2α and VEGF-A mRNA was observed in OMA. The overall data point out that the heterogeneity of the disease reflects differences in expression levels of genes associated with hypoxia and angiogenesis, suggesting that such conditions may have an active role in the development of the disease.

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Authors and Affiliations

  1. Cellular and Molecular Physiology Unit, Department of Molecular and Developmental Medicine, University of Siena, Siena, Italy

    Irene Filippi PhD & Antonella Naldini PhD

  2. Obstetrics and Gynecology Unit, Department of Molecular and Developmental Medicine, “S. Maria alle Scotte” Hospital, University of Siena, University of Siena, Viale Mario Bracci, 1, 53100, Siena, Italy

    Patrizia Carrarelli PhD, Stefano Luisi MD, PhD & Felice Petraglia MD

  3. Department of Immunology, Hôpital Cochin, Assistance Publique-Hôpitaux de Paris, Paris, France

    Frederic Batteux MD, PhD

  4. Université Paris Descartes, Sorbonne Paris Cité, Inserm, Unité de Recherche, Institut Cochin, CNRS, Paris, France

    Frederic Batteux MD, PhD & Charles Chapron MD

  5. Department of Gynecology Obstetrics II and Reproductive Medicine, Université Paris Descartes, Sorbone Paris Cité, Faculté de Médecine, Assistance Publique-Hôpitaux de Paris, Groupe Hospitalier Universitaire Ouest, Centre Hospitalier Universitaire Cochin, Paris, France

    Charles Chapron MD

Authors
  1. Irene Filippi PhD
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  2. Patrizia Carrarelli PhD
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  3. Stefano Luisi MD, PhD
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  4. Frederic Batteux MD, PhD
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  5. Charles Chapron MD
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  6. Antonella Naldini PhD
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  7. Felice Petraglia MD
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Correspondence to Felice Petraglia MD.

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Filippi, I., Carrarelli, P., Luisi, S. et al. Different Expression of Hypoxic and Angiogenic Factors in Human Endometriotic Lesions. Reprod. Sci. 23, 492–497 (2016). https://doi.org/10.1177/1933719115607978

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