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Analysis of Serum microRNA Profile by Solexa Sequencing in Women With Endometriosis

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Abstract

Objective(s)

The potential roles of serum microRNAs (miRNAs), as biomarkers, in noninvasive diagnosis of endometriosis have been reported by microarray analysis. However, microarray analysis cannot perform well in outcome accuracy and repeatability and is not suitable to be used for exploring new targets. Here, Solexa sequencing, a wide and precise method, was adopted to further analyze the serum miRNAs profile in endometriosis, which may offer more evidence to apply serum miRNAs as biomarkers in diagnosis of endometriosis.

Materials and Methods

Serum samples were collected from 30 patients with minimal–mild endometriosis and 20 women without endometriosis as control. Expression of serum miRNAs was measured by Solexa sequencing and validated by quantitative real-time polymerase chain reaction (qPCR).

Results

Solexa sequencing showed 93.63% clean readouts for all small RNAs in the serum of patients with endometriosis and controls. A total of 108 miRNAs were found to be differentially expressed in the serum of patients with endometriosis by deep sequencing, compared to controls. Among them, 98 miRNAs were significantly downregulated, while 10 miRNAs were significantly upregulated. Only 21 of 98 significantly downregulated miRNAs, and none of significantly upregulated miRNAs were reported in published literatures, which may be due to the differences in samples and analytical methods. The Solexa sequencing results were consequently validated by qPCR in additional samples. Some miRNAs were identified to be promising diagnostic markers of endometriosis. The functional annotation of target genes revealed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses indicated that a majority of differential miRNAs might be involved in endometriosis.

Conclusion

Circulating miRNAs may be useful as detection biomarkers for the early diagnosis of minimal–mild endometriosis.

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References

  1. Nisolle, M, Donnez, J Peritoneal endometriosis, ovarian endome-triosis, and adenomyotic nodules of the rectovaginal septum are three different entities. Fertil Steril. 1997;68(4):585–596.

    CAS  PubMed  Google Scholar 

  2. Matsuzaki, S, Maleysson, E, Darcha, C Analysis of matrix metalloproteinase-7 expression in eutopic and ectopic endome-trium samples from patients with different forms of endometrio-sis. Hum Reprod. 2010;25(3):742–750.

    CAS  PubMed  Google Scholar 

  3. Fadhlaoui, A, Gillon, T, Lebbi, I, Bouquet de Jolinière, J, Feki, A Endometriosis and Vesico-Sphincteral Disorders. Front Surg. 2015;2:23.

    PubMed  PubMed Central  Google Scholar 

  4. Bagga, S, Pasquinelli, AE Identification and analysis of micro-RNAs. Genet Eng. 2006;27:1–20.

    CAS  Google Scholar 

  5. Lam, EW, Shah, K, Brosens, JJ The diversity of sex steroid action: the role of micro-RNAs and FOXO transcription factors in cycling endometrium and cancer. J Endocrinol. 2012;212(1): 13–25.

    CAS  PubMed  Google Scholar 

  6. He, Y, Lin, J, Kong, D, et al. Current state of circulating Micro-RNAs as cancer biomarkers. Clin Chem. 2015;61(9): 1138–1155.

    CAS  PubMed  Google Scholar 

  7. Burney, RO, Hamilton, AE, Aghajanova, L, et al. MicroRNA expression profiling of eutopic secretory endometrium in women with versus without endometriosis. Mol Hum Reprod. 2009; 15(10):625–631.

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Wang, WT, Zhao, YN, Han, BW, et al. Circulating microRNAs identified in a genome-wide serum microRNA expression analysis as noninvasive biomarkers for endometriosis. J Clin Endocr Metab. 2013;98(1):281–289.

    CAS  PubMed  Google Scholar 

  9. Jia, SZ, Yang, Y, Lang, J, Sun, P, Leng, J Plasma miR-17-5p, miR-20a and miR-22 are down-regulated in women with endometrio-sis. Hum Reprod. 2013;28(2):322–330.

    CAS  PubMed  Google Scholar 

  10. Cho, S, Mutlu, L, Grechukhina, O, Taylor, HS Circulating micro-RNAs as potential biomarkers for endometriosis. Fertil Steril. 2015;103(5):1252–1260.

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Xu, P, Wang, L, Huang, L, et al. Identification and characterization of microRNAs expressed in human breast cancer chemo-resistant MCF-7/Adr cells by Solexa deep-sequencing technology. Biomed Pharmacother. 2015;75:173–178.

    CAS  PubMed  Google Scholar 

  12. Hafner, M, Landgraf, P, Ludwig, J, et al. Identification of micro-RNAs and other small regulatory RNAs using cDNA library sequencing. Methods. 2008;44(1):3–12.

    CAS  PubMed  PubMed Central  Google Scholar 

  13. McDonald, JS, Milosevic, D, Reddi, HV, Grebe, SK, Algeciras-Schimnich, A Analysis of circulating microRNA: preanalytical and analytical challenges. Clin Chem. 2011;57(6):833–840.

    CAS  PubMed  Google Scholar 

  14. Hauser, S, Wulfken, LM, Holdenrieder, S, et al. Analysis of serum microRNAs (miR-26a-2*, miR-191, miR-337-3p and miR-378) as potential biomarkers in renal cell carcinoma. Cancer Epidemiol. 2012;36(4):391–394.

    CAS  PubMed  Google Scholar 

  15. Chen, X, Ba, Y, Ma, L, et al. Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases. Cell Res. 2008;18(10):997–1006

    CAS  PubMed  Google Scholar 

  16. Wheeler, JM Epidemiology of endometriosis-associated infertility. J Reprod Med. 1989;34(1):41–46.

    CAS  PubMed  Google Scholar 

  17. Ozkan, S, Murk, W, Arici, A Endometriosis and infertility: epide-miology and evidence-based treatments. Ann N Y Acad Sci. 2008; 1127:92–100.

    PubMed  Google Scholar 

  18. Filigheddu, N, Gregnanin, I, Porporato, PE, et al. Differential expression of microRNAs between eutopic and ectopic endometrium in ovarian endometriosis. J Biomed Biotechnol. 2010;2010:369549.

    PubMed  PubMed Central  Google Scholar 

  19. Pan, Q, Luo, X, Toloubeydokhti, T, Chegini, N The expression profile of micro-RNA in endometrium and endometriosis and the influence of ovarian steroids on their expression. Mol Hum Reprod. 2007;13(11):797–806.

    CAS  PubMed  Google Scholar 

  20. Zhang, J, Liu, Y, Liu, Z, et al. Differential expression profiling and functional analysis of microRNAs through stage I-III papillary thyroid carcinoma. Int J Med Sci. 2013;10(5):585–92.

    CAS  PubMed  PubMed Central  Google Scholar 

  21. Li, J, Liu, Y, Wang, C, et al. Serum miRNA expression profile as a prognostic biomarker of stage II/III colorectal adenocarcinoma. Sci Rep. 2015;5:12921.

    CAS  PubMed  PubMed Central  Google Scholar 

  22. Shen, H, Chen, HY, Jia, B, Han, GH, Zhang, YS, Zeng, XC Char-acterization and expression analysis of microRNAs in Qira black sheep and Hetian sheep ovaries using Solexa sequencing. Genet Mol Res. 2015;14(3):7356–7367.

    CAS  PubMed  Google Scholar 

  23. Ahn, SH, Monsanto, SP, Miller, C, Singh, SS, Thomas, R, Tayade, C Pathophysiology and immune dysfunction in endometriosis. Biomed Res Int. 2015;2015:795976.

    PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Key Laboratory for Carcinogenesis of Chinese Ministry of Health, Cancer Research Institute, School of Basic Medical Science, Central South University, Changsha, Hunan, China

    Lei Wang MS & Caiping Ren MD

  2. Research Center of Carcinogenesis and Targeted Therapy, Xiangya Hospital, Central South University, Changsha, Hunan, China

    Wei Huang MD

  3. The Higher Educational Key Laboratory for Cancer Proteomics and Translational Medicine of Hunan Province, Xiangya Hospital, Central South University, Changsha, Hunan, China

    Wei Huang MD

  4. Department of Dermatology, Hunan Key Laboratory of Medical Epigenomics, Second Xiangya Hospital, Central South University, Changsha, Hunan, China

    Ming Zhao MD

  5. Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan, China

    Xingjun Jiang MD

  6. Department of Obstetrics and Gynecology, Second Xiangya Hospital, Central South University, 139 Renmin Rd, Changsha, Hunan, 410011, China

    Xiaoling Fang MD & Xiaomeng Xia MD

Authors
  1. Lei Wang MS
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  2. Wei Huang MD
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  3. Caiping Ren MD
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  4. Ming Zhao MD
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  5. Xingjun Jiang MD
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  6. Xiaoling Fang MD
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  7. Xiaomeng Xia MD
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Corresponding author

Correspondence to Xiaomeng Xia MD.

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Wang, L., Huang, W., Ren, C. et al. Analysis of Serum microRNA Profile by Solexa Sequencing in Women With Endometriosis. Reprod. Sci. 23, 1359–1370 (2016). https://doi.org/10.1177/1933719116641761

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  • DOI: https://doi.org/10.1177/1933719116641761

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