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The Effect of Catalase on The Toxicity of Cadmium in Cadmium-Sensitive and Cadmium-Resistant Staphylococcus Aureus

Effekten av katalas på kadmiums toxicitet på kadmiumkånslig och kadmiumresistent Staphylococcus aureus.

Acta Veterinaria Scandinavica volume 21, pages 209–223 (1980)Cite this article

Abstract

The effect of catalase on the toxicity of cadmium (Cd) in Cd-resistant Staphylococcus aureus 3719+ and its plasmid-negative Cd-sensitive variant 3719– was studied. Catalase on a solid medium increased the recovery of Gd-stressed S. aureus 3719– cells, and the addition of catalase into a liquid growth medium resulted in a shortened lag phase of growth especially in S. aureus 3719–. The catalase activity of S. aureus 3719+ cell suspensions was greater than the corresponding activity of S. aureus 3719– cell suspensions. Cd did not influence the activity of beef liver catalase or the catalase production of the bacterial cells.

Catalase reduced the toxicity of Cd especially for S. aureus 3719–. The greater catalase production of S. aureus 3719+ might be one factor in its resistance against the toxic effects of Cd. It is suggested that Cd together with hydrogen peroxide may induce oxidative damage to cells if there is not sufficient catalase available to decompose all the hydrogen peroxide formed.

Sammanfattning

Effekten av katalas på kadmiums (Cd) toxicitet på en Cd-resistent Staphylococcus aureus stam 3719+, och dess plasmid-negativa, Cd-kånsliga variant 3719– undersoktes. Enzymet katalas på ett fast substrat ökade cellantalet av den Gd-belastade S. aureus 3719– stammen. Tillsatsen av katalas till det flytande nöringssubstratet förkortade lagfasen särskilt i S. aureus 3719–. Katalasaktiviteten i S. aureus 3719-f- cellsuspensionerna var större än den motsvarande aktiviteten i S. aureus 3719– cellsuspensionerna. Cd påverkade inte aktiviteten av katalas från nötlever eller katalasproduktionen av bakteriecellerna.

Katalas minskade Cd:s toxicitet på specielt S. aureus 3719–. Den större katalasproduktionen av S. aureus 3719+ kunde vara en av dess resistensfaktorer mot Gd:s toxiska effekt. Det är möjligt, att Cd till-sammans med väteperoxid kunde förorsaka en oxidativ skada på celler om det inte finns tillräckligt med katalas at nedbryta den producerade väteperoxiden.

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Authors and Affiliations

  1. Department of Food Hygiene, College of Veterinary Medicine, Helsinki, Finland

    H. Korkeala & Satu Sankari

  2. Department of Biochemistry, College of Veterinary Medicine, Helsinki, Finland

    H. Korkeala & Satu Sankari

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  1. H. Korkeala
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  2. Satu Sankari
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Korkeala, H., Sankari, S. The Effect of Catalase on The Toxicity of Cadmium in Cadmium-Sensitive and Cadmium-Resistant Staphylococcus Aureus. Acta Vet Scand 21, 209–223 (1980). https://doi.org/10.1186/BF03546884

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Acta Veterinaria Scandinavica

ISSN: 1751-0147