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Plasma Progesterone Assays in Superovulated Cattle
Plasmaprogesteronbestemmelser ved superovulation af kvseg
Acta Veterinaria Scandinavica volume 19, pages 298–309 (1978)
Abstract
Plasma progesterone was measured in 14 normally cycling heifers and cows subjected to non-surgical recoveries of embryos. A radioimmunoassay (RIA) method was used for progesterone determination. The average progesterone concentration increased from 7.5 to 11.6 ng/ml in 8 of the animals following treatment with PMSG on day 8–12. Six animals had a decrease from 5.0 ± 2.1 to 3.9 ± 2.5 ng/ml. The overall increase was from 6.4 ± 2.7 ng/ml to 8.3 ± 4.8 ng/ ml. Prostaglandin F2a-analogue (cloprostenol) treatment resulted in a sharp decrease in plasma progesterone followed by a rapid increase to an average of 46.8 ng/ml on day 16. A high degree of variability in this peak value was observed, and it was not correlated with the number of corpora lutea. The superovulatory cycle was generally prolonged. The heat following the superovulatory treatment was silent, and a typical ovarian resting period was observed during which the progesterone concentration remained low and the ovaries small.
Sammendrag
Plasmaprogesteron blev målt på 14 normale kvier og køer, der anvendtes som don örer ved ikke-kirurgiske fostertransplantationer. Progesteronbestemmelserne udførtes med en radioimmunoassay (RIA) metode. Progesteronværdierne steg efter behandling med PMSG fra gns. 6,4 ± 2,7 ng/ml til 8,3 ± 4,8 ng/ml. Hos 8 af dyrene forekom der en stigning fra gns. 7,5 ± 2,8 til 11,6 ± 3,1 ng/ml. Hos de øvrige 6 dyr konstateredes et fald fra gns. 5,0 ± 2,1 til 3,9 ± 2,5 ng/ml. Gloproste- nolbehandling 2 dage senere resulterede i et markant fald efterfulgt af en hurtig stigning op til gennemsnitligt 46,8 ng/ml på dag 16. Der var en betydelig individuel variation i maksimum værdierne, og der observeredes ingen sammenhæng mellem disse og antallet af corpora lutea. I visse tilfælde var den superovulerede brunstcyklus af forlæn- get varighed. Den brunst, som optrådte efter superovulationen, efter- fulgtes af en længere ovarial hvileperiode med meget lave plasma- progesteronværdier ( ⩽ 1 ng/ml) og ingen fø1elig ovarial aktivitet.
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Solti, L., Greve, T. & Koefoed-Johnsen, H.H. Plasma Progesterone Assays in Superovulated Cattle. Acta Vet Scand 19, 298–309 (1978). https://doi.org/10.1186/BF03547634
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DOI: https://doi.org/10.1186/BF03547634