Journal of Lipid Research
Research ArticlesDepletion of essential isoprenoids and ER stress induction following acute liver-specific deletion of HMG-CoA reductase
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Author contributions—M.D.G. and W.R.L. conceptualization; M.D.G. and W.R.L. design; M.D.G. experiments; M.D.G. analysis; A.H. viral vector production; K.E.J., J.C.B., A.M.D., A.H., A.L., R.H.H., and M.F. technical and intellectual support; K.R.P. transmission electron microscopy data analysis; K.R.P. pathological scores; J.H. and C.H.B. lipidomics; M.D.G. and W.R.L. writing-original draft; M.D.G., K.E.J., J.C.B., A.M.D., A.H., A.L., R.H.H., M.F., K.R.P., J.H., C.H.B, and W.R.L. writing-editing and review.
Author ORCIDs—Christoph H. Borchers http://orcid.org/0000-0003-2394-6512; William R. Lagor http://orcid.org/0000-0002-1703-5125
Funding and additional information—This work was supported by the National Institutes of Health (HL132840 and DK124477 to W.R.L.; T32HL07676 and T32GM08321 to K.E.J.; T32HL07676 to A.M.D.; and R01-HL132840-02S1 to M.F.), the American Heart Association (19POST34430092 to M.D.G. and 19PRE34380467 to A.M.D.). This work was also supported by the Texas Digestive Diseases Morphology Core (P30DK56338) and the Integrated Microscopy Core (DK56338 and CA125123). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Conflict of interest—The authors declare that they have no conflicts of interest with the contents of this article.
Abbreviations—
- Aat
α-1 antitrypsin
- ALP
alkaline phosphatase
- ALT
alanine aminotransferase
- Dhdds
dehydrodolichyl diphosphate synthase subunit
- DKO
double KO
- GC
genome copy
- gRNA
guide RNA
- Hmgcr
HMG-CoA reductase
- HLP
hybrid liver-specific promoter
- Insig
insulin-induced gene
- LSKO
liver-specific KO
- PDSS
prenyl diphosphate synthase
- SaCas9
Staphylococcus aureus Cas9
Manuscript received June 26, 2020, and in revised form October 12, 2020. Published, JLR Papers in Press, October 27, 2020, DOI 10.1194/jlr.RA120001006.