ABSTRACT
This chapter discusses in detail some of the prominent microfluidic strategies employed for cellular manipulation, analysis, and treatment and their feasibility in clinical applications. The major advantage of microfluidic techniques over other conventional methods is that they provide high-throughput devices for the manipulation and analysis of cells. Many high-throughput microfluidic devices have been used for single-cell trapping using electro-osmotic flow manipulation, microvortex manipulation, etc. The chapter discusses microfluidic-based physical techniques, such as electroporation and mechanoporation, for single-cell therapeutic and diagnostic purposes. Droplet microfluidics is frequently employed for single-cell analysis due to its high specificity and potentiality to isolate single cells. Additionally, microfluidic technologies are widely used to analyze single-cell intracellular components, such as DNA, RNA, proteins, and amino acids. Analysis of these components on a single-cell level is important due to the extreme variation in the composition of proteins within a population of cells.
