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Preparation and Evaluation of Miconazole Nitrate-Loaded Solid Lipid Nanoparticles for Topical Delivery

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Abstract

The purpose of this study was to prepare miconazole nitrate (MN) loaded solid lipid nanoparticles (MN-SLN) effective for topical delivery of miconazole nitrate. Compritol 888 ATO as lipid, propylene glycol (PG) to increase drug solubility in lipid, tween 80, and glyceryl monostearate were used as the surfactants to stabilize SLN dispersion in the SLN preparation using hot homogenization method. SLN dispersions exhibited average size between 244 and 766 nm. All the dispersions had high entrapment efficiency ranging from 80% to 100%. The MN-SLN dispersion which showed good stability for a period of 1 month was selected. This MN-SLN was characterized for particle size, entrapment efficiency, and X-ray diffraction. The penetration of miconazole nitrate from the gel formulated using selected MN-SLN dispersion as into cadaver skins was evaluated ex-vivo using franz diffusion cell. The results of differential scanning calorimetry (DSC) showed that MN was dispersed in SLN in an amorphous state. The MN-SLN formulations could significantly increase the accumulative uptake of MN in skin over the marketed gel and showed a significantly enhanced skin targeting effect. These results indicate that the studied MN-SLN formulation with skin targeting may be a promising carrier for topical delivery of miconazole nitrate.

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Acknowledgements

The authors would like to thank Mr. Harish Rao of Serum India Ltd. for his kind help with ultracentrifugation. We are also thankful to Gattefosse, France, for kindly supplying the lipids used in this study and Rajadilip Gupta of Glenmark Laboratories for his assistance.

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Authors and Affiliations

  1. AISSMS College of Pharmacy, Kennedy Road, Pune, 411001, India

    Mangesh R. Bhalekar, Ashwini Madgulkar, Nilam Patil & Nilkanth Patil

  2. Bharati Vidyapeeth University, Poona College of Pharmacy, Erandwane, Pune, 411038, India

    Varsha Pokharkar

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  1. Mangesh R. Bhalekar
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Correspondence to Mangesh R. Bhalekar.

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Bhalekar, M.R., Pokharkar, V., Madgulkar, A. et al. Preparation and Evaluation of Miconazole Nitrate-Loaded Solid Lipid Nanoparticles for Topical Delivery. AAPS PharmSciTech 10, 289–296 (2009). https://doi.org/10.1208/s12249-009-9199-0

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