From the fronds of Diplazium subsinuatum (WALL.) TAGAWA, a new triterpene lactone (I) and its glycosides II, III, IV were isolated. The structure of I was established as 17, 24-dihydroxyhopan-28, 22-olide on the basis of spectral data and chemical conversions. The glycoside II was identified as 17-hydroxy-24-O-β-D-glucopyranosyl-hopan-28, 22-olide, III as 17-hydroxy-24-O-[α-L-arabinofuranosyl-(1→2)]-β-D-glucopyranosyl-hopan-28, 22-olide and IV as 17-hydroxy-24-O-[α-L-arabinofuranosyl-(1→2)]-[β-D-glucopyranosyl-(1→6)]-β-D-glucopyranosyl-hopan-28, 22-olide by spectroscopic analysis and hydrolysis reactions.