Six new highly oxygenated lanostane-type triterpenes, called ganoderic acid γ(1), ganoderic acid δ(2), ganoderic acid ε(3), ganoderic acid ζ(4), ganoderic acid η(5) and ganoderic acid θ(6), were isolated from the spores of Ganoderma lucidum, together with known ganolucidic acid D (7) and ganoderic acid C2 (8). Their structures of the new triterpenes were determined as (23S)-7β, 15α, 23-trihydroxy-3, 11-dioxolanosta-8, 24(E)-diene-26-oic acid (1), (23S)-7α, 15α, 23-trihydroxy-3, 11-dioxolanosta-8, 24(E)-diene-26-oic acid (2), (23S)-3β, 7β, 23-trihydroxy-11, 15-dioxolanosta-8, 24(E)-diene-26-oic acid (3), (23S)-3β, 23-dihydroxy-7, 11, 15-trioxolanosta-8, 24(E)-diene-26-oic acid (4), (23S)-3β, 7β, 12β, 23-tetrahydroxy-11, 15-dioxolanosta-8, 24(E)-diene-26-oic acid (5) and (23S)-3β, 12β, 23-trihydroxy-7, 11, 15-trioxolanosta-8, 24(E)-diene-26-oic acid (6), respectively, by chemical and spectroscopic means, which included the determination of a chiral center in the side chain by a modification of Mosher's method. The cytotoxicity of the compounds isolated from the Ganoderma spores was carried out in vitro against Meth-A and LLC tumor cell lines.