Two saponins (I) and (II) which are detected by conventional paper chromatography of the seed saponins of Digitalis purpurea L. (commercial "digitonin") were isolated by column chromatography on alumina and it was found that I is composed of three tetraglycosides, desglucotigonin, gitonin (m.p. 238∼241°(decomp.), [α]²0_D-60.0°) and desglucodigitonin (m.p. 243∼245°(decomp.), [α]²0_D-71.4°), while II is a mixture of two pentaglycosides, digalonin and digitonin.