In this article, computational investigation of the gas–liquid flow in stirred tank with multiple Rushton impellers was carried out. Experimental results of average and local details of gas–liquid flow characters were reported, including gas–holdup, bubble Sauter diameter, specific area and mass transfer coefficient. Thoroughly interphase momentum transfer models including drag and non-drag models were investigated, together with four mass transfer coefficient models using commercial CFD software ANSYS CFX 12.0. Among the drag models tested, the modified Brucato model showed more accurate results than others, while non-drag models showed slight effects on the average flow and local flow characters. For accurate local flow simulation, the population balance model (PBM) showed quite sensitive effect on the local gas distribution. Simulation of interphase mass transfer with eddy cell model showed the most agreement with experimental measured data. Additionally, two advection schemes were employed and compared with each other to test their ability to get more accurate simulation result.