Calluses were induced with 100% rate from anthers of 8 species and 2 varieties of diploid and polyploid Chrysanthemum species.
Most of the calluses were found to be derived from the cells of the connective tissue between the thecae of the anthers. Within 40 days from cultivation, these calluses differentiated roots, and numerous shoots were differentiated within 40 days after transplanting to the medium without auxin hormones.
The induced plants examined in some genetic strains showed the same chromosome number as the respective mother strains.