Genetic analysis of plant regeneration ability in rice was studied in 14 x 14 diallel crosses using 4 japonica, 4 javanica and 6 indica varieties. Calli were obtained from mature seeds and regenerated plantlets were induced in suspension cultures. Among the 14 varities, Kele showed the highest regeneration ability. The largest number of regenerated plantlets was obtained in the F1 hybrid of Kele × Banten. No regenerated plantlet was observed in 4 parents and 8 Fls. The relationship between Vr and Wr in the lO X 10 subdiallel table suggested that there was no non-allelic interaction and that the low regeneration ability was dominant. Analysis of variance of lo ×10 diallel table showed that both additive and dominant effects were significant, and reciprocal effect was also significant. There was no significant relationship between ecospecies and above genetic properties. Among the 14 varieties, indica variety, Qing Er Ai and Kele would be useful to improve the re-generation ability, because of their significant high GCA and favorable maternal effects. Heterosis of regeneration ability in the crosses of japouica x indica, javauica x indica and their respective reciprocal crosses was higher than the crosses in japonica × japonica, japonica x javanica and javanica x japonica . There was no significant correlation (r = 0.199) between regeneration ability and shoot dry weight of each F₁ hybrid, indicating that it was difficult to obtain the F1 hybrids showing high heter-osis in both characters.