It was confirmed that turbid substances which remained in saccharified liquid after digestion of starch slurry with bacterial alpha-amylase and glucoamylase were insoluble starch particles and that these materials were formed during heat treatment of the slurry in liquefaction process.
In iodine staining reaction, the suspension of these materials showed only u slightly dirty blue color; even after boiling of this suspension for 30 min at 135°C, this staining reaction was not complete and the maximum color intensity could be obtained after treat-ment with 1 N caustic soda solution.
It is assumed from the iodine color reaction, that the main component of these materials was glucose polymer of straight chain, like that of amylose, and that this would be strongly associated with each other to form the compact molecule.
The method for determination of these insoluble starch particles in the starch slurry was established and by this method the contents of these materials were determined in some types of starches.

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