Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
Chitin Coated Cellulose as an Adsorbent of lysozyme-like Enzymes Preparation and Properties
Taiji IMOTOKazuyoshi YAGISHITA
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1973 Volume 37 Issue 3 Pages 465-470

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Abstract

As a new adsorbent of lysozyme-like enzymes, chitin coated (CC-)cellulose was prepared. CC-cellulose was stable and had good flow properties for use in column chromatography. Affinity chromatography with CC-cellulose showed that 3_??_5mg of lysozyme/ml resin was adsorbed specifically and desorbed quantitatively under mild conditions. The utilities of the method of affinity chromatography with CC-cellulose are discussed.

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