Establishment of a new method for preparation of xylobiose was investigated.
When hardwood xylan was hydrolyzed by the xylanase system of Streptomyces sp. E-86, the hydrolyzate mainly consisted of xylose and xylobiose in an approximate ratio of 1:1.
Yeast strains selected in this paper, effectively and selectively metabolized all of xylose without any degradation of xylobiose.
By successive treatments of xylan with Streptomyces xylanase system and xylose-metabolizable yeast, 70.4g of crystalline xylobiose was obtained from 172g of xylan.

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